c-Fos参与羊驼小肠黏膜淋巴细胞生发中心的调控

c-Fos Involvement in the Regulation of Mucosa Lymphocyte Germinal Center of Small Intestine in Alpaca (lama pacos)

【目的】研究转录因子c-Fos与凋亡相关蛋白Caspase3在羊驼小肠黏膜淋巴组织的表达,探索c-Fos与羊驼小肠黏膜淋巴细胞生发中心的关系。【方法】用免疫组织化学技术研究c-Fos蛋白与Caspase3蛋白在羊驼十二指肠和空肠淋巴组织的表达。【结果】免疫组织化学结果显示羊驼十二指肠黏膜淋巴细胞较少,c-Fos蛋白主要表达于空肠黏膜下淋巴组织生发中心,在黏膜下淋巴组织向固有层淋巴组织过渡区域有散在表达,在固有层弥散淋巴组织中c-Fos阳性表达细胞较少。Caspase3蛋白主要表达于空肠黏膜固有层淋巴组织,在黏膜下淋巴组织生发中心很少表达,在黏膜下淋巴组织向固有层弥散淋巴组织过渡区域Caspase3有散在表达。【结论】c-Fos参与羊驼空肠黏膜下淋巴组织生发中心增殖与分化的调控。

[Objective] To study the expression of c-Fos and Caspase3 in small intestine lymphoid tissue of alpaca, and to explore the function of c-Fos in the regulation of mucosa lymphocyte germinal center of alpaca small intestine. [Method] The distribution of c-Fos and Caspase3 in small intestine lymphoid tissue in alpaca were studied by immunohistochemistry in the present study. [ Result ] The results showed that few lymphocyte was found in the mucosa of duodenum, the c-Fos positive lymphocytes were located mainly in the germinal center of jejunum Peyer＇s patches, a little c-Fos positive cells were found in the lamina propria of jejunum and c-Fos positive cells could be seen in the region of adjacent lamina propria. The expression of Caspase3 was focused in the lamina propria lymphocytes. There were almost no Caspase3 positive cells in the germinal center of Peyer＇s patches. A few Caspase3 positive cells were located in the region adjacent lamina propria. [Conclusion] Based on these findings, it is concluded that c-Fos involves in the regulation of mucosa lymphocyte germinal center of small intestine proliferation and differentiation in alpaca.