摘要
氟喹诺酮类(Fluoroquinolones,FQs)各单体药物之间的化学结构极其类似,通常针对某一个药物制备的抗体对多种氟喹诺酮具有广谱识别性,是易于建立多残留免疫分析的代表性药物。本文综述了氟喹诺酮抗体制备和食品中的免疫分析研究进展,分析了已发表文章中以不同氟喹诺酮为半抗原所获得抗体对其它氟喹诺酮的交叉反应,并应用SYBYL.7.0程序包以MMFF94力场优化了8种批准用于动物的氟喹诺酮最低能量构象,从二维和三维构象角度对制备广谱氟喹诺酮抗体的策略进行了探讨,理论上提出了制备广谱氟喹诺酮抗体的途径。
The antibody against one of the fluoroquinolones （FQs） alsways possesses broad-spectrum binding characteristics to FQs due to the similarity between individual drugs of FQs, which are easy to be detected by multi-residue immunoassay. This paper reviewed advances in the development of immunoassay for FQs in food samples and analyzed the cross-reaction results from papers published, optimized the minimum energy conformations of 8 FQs approved to be used in animals. A theoretic strategy for obtaining broad-spectrum antibody was put forward based on the evidence of two-dimensional and three-dimensional view.
出处
《中国农业科学》
CAS
CSCD
北大核心
2008年第10期3311-3317,共7页
Scientia Agricultura Sinica
基金
国家自然科学重点基金(30830082)
北京市优秀博士论文基金(YB20081001902)
中国农业大学科研启动基金(2007024)
关键词
氟喹诺酮
广谱抗体
免疫分析
分子模拟
Fluoroquinolone
Broad-spectrum antibody
Immunoassay
Molecular modeling
