摘要
【目的】以玉米第二真叶下表皮为材料,研究ABA诱导的保卫细胞胞质Ca2+浓度的变化,并探讨Ca2+来源。【方法】通过数字激光共聚焦显微镜测定ABA诱导的胞质钙离子浓度变化,并通过异搏啶、EGTA[乙二醇双(2--氨基)乙基醚-N,N,N’,N’四乙酸]药理实验探讨钙离子的来源。【结果】ABA不能诱导所有的保卫细胞胞质钙离子浓度的增加,而可被ABA诱导的保卫细胞中又表现了不同的钙离子浓度变化形式。异搏啶预处理后,不能改变保卫细胞对ABA的反应,但EGTA预处理,则抑制了ABA诱导的胞质钙离子浓度升高。此外,气孔运动观察结果显示:ABA可以明显的诱导气孔关闭,但EGTA、异搏啶的预处理延缓了ABA诱导的气孔关闭速度。【结论】ABA作用下,保卫细胞胞质Ca2+浓度或不发生变化,或持续高浓度,或发生逐渐升高,形成不同的钙信号,最终造成气孔不同步关闭;ABA诱导的胞质钙离子升高主要源自胞外钙离子内流。
[Objective] The change of cytosolic calcium concentration induced by ABA and the origin of calcium in maize pre-treated with EGTA and verapamil are measured. [Method] The changes of cytosolic calcium concentration were studied by confocal laser scanning microscopy, and the origin of calcium using EGTA or verapamil. [Result] The addition of 50 μmol·L^-1 ABA to guard cells did not elicit the increase, or gradual increase or sustained increase in [Ca^2＋]cyt, and showed that the guard cells displayed different sensitivity in response to ABA. Compared with ABA trials, ABA elicits no different reactions after pretreated with verapamil for 30 min, but the elevation of [Ca^2＋]cyt was suppressed after pretreated with EGTA. At the same time, the result of stomatal movements showed that ABA may obviously induce the closure of the stoma, and pre-treated with EGTA or verapamil, closure of the stoma induced by ABA was delayed. [ Conclusion ] The various change of cytosolic calcium induced by ABA caused the asynchronous closure of stoma, and the increase of cytosolic calcium was mainly the result of influx of calcium.
出处
《中国农业科学》
CAS
CSCD
北大核心
2008年第10期3357-3362,共6页
Scientia Agricultura Sinica
基金
河北省自然科学基金项目(C2007000994,C200600727)
