苦马豆素对小鼠腹腔巨噬细胞免疫功能的影响

Effects of Swainsonine on Immune Function of Peritoneal Macrophages in Mice

【目的】探索苦马豆素(SW)与小鼠腹腔巨噬细胞功能之间的关系,为SW免疫研究提供新的试验依据。【方法】将70只小鼠随机分为7组,其中5组分别按0.05、0.2、0.8、3.2和6.4mg·kg-1每天灌服SW生理盐水,连续21d,另2组为生理盐水和空白对照组。收集小鼠腹腔巨噬细胞,经脂多糖(LPS)诱导活化,用ELISA法检测TNF-α生成,酶法检测一氧化氮合酶(NOS)活性,化学法检测H2O2浓度,MTT比色法检测杀伤肿瘤细胞活性及吞噬中性红能力。【结果】SW剂量在0.2～3.2mg·kg-1之间,能够提高小鼠腹腔巨噬细胞的吞噬和杀伤活性﹑NOS活性,促进TNF-α和H2O2生成,且均呈剂量效应关系;6.4mg·kg-1SW对小鼠巨噬细胞的活性具有抑制作用。【结论】低剂量SW可以通过提高NOS活性介导巨噬细胞信号传递通路,促进TNF-α和H2O2生成,发挥其吞噬和杀伤功能;高剂量SW对小鼠巨噬细胞免疫功能起抑制作用。提示,SW对小鼠腹腔巨噬细胞具有双向调节作用。

[ Objective ] The effects of swainsonine （SW） on immune function of peritoneal macrophages in mice were studied in order to provide new test results. [Method] Seventy mice were divided into 7 groups randomly. The animals in five groups were administrated orally with 0.05, 0.2, 0.8, 3.2 and 6.4 mg·kg^-1 SW/d separately for 21 days. One group was administrated with physiologic saline solution and the other one as a control group. Peritoneal macrophages were collected and activated by LPS. TNF-α was examined by ELISA. The activity of nitric oxide synthase （NOS） and the release of H2O2 were measured by enzymic and chemical methods. MTT colorimetry was used to determine the capability of phagocytosis neutral red and killing tumor cell. [ Result ] SW increased the activitis of phagocytosis, the killing tumor cell and NOS, promoted the release of H2O2 and TNF-α at a dosage of 0.2-3.2 mg·kg^-1 with dose-effect relation. SW inhibited the activation of macrophage at a the dosage of 6.4 mg·kg^-1. [Conclusion] SW can perform its immune function via signal transduction system by activating macrophages to raise the activity of NOS so as to modulate the level of TNF-α and to release H2O2, SW at a dosage of 6.4 mg·kg^-1 can depress the immune function of macrophages. These results indicated that SW can influence immune function of peritoneal macrophages by two-way regulation.