制霉菌素在义齿软衬材料中抗菌性研究

Nystatin enhances antifungal ability of soft lining materials

目的探讨在软衬材料中加入抗真菌药物制霉菌素后对材料抗菌性的影响。方法实验选用了梅卡硅橡胶室温固化软衬材料、COESOFT软衬材料、日进软衬材料、上海软衬垫等四种材料,抗真菌药物制霉菌素分别按0%,3%,6%,9%(W/W)药物/试件比例加入各种材料中,分别制成标准试件。通过培养方法监测对白色念珠菌标准株的抗菌性及时相关系。结果在4种材料中,加入制霉菌素试件对念珠菌的抑制性能较之同种未加入制霉菌素者后均增加,具有统计学差异。不同材料中加入药物后,其抗菌性有一定的时间差异,但都在第6～8天后抗菌性明显减低。6%W/W药物材料比例试件测试结果与9%差异无统计学意义,与无药物材料和3%比较,抗菌效果增加。结论无论何种材料,药物浓度均与念珠菌生长抑制作用相关,而随着时间的推移,高浓度药物的材料对念珠菌生长抑制作用衰退较慢,但一定时间后,药物作用无明显差异,6%W/W药物材料比例即可达到有效抑菌作用。在软衬材料中加入制霉菌素可作为暂时性软衬材料短期使用时预防义齿性口炎的积极措施。

[Objective]Soft lining materials play an important role in chnical removable denture, however, there is an increased probability of yeast colonization on soft lining materials, which is a causative factor in denture stomatitis. This study aimed to investigate the function of addition of Nystatin into soft lining material for anti-candida adherence. [Methods] Four different types of soft lining materials were used in this study. Nystatin was added into materials respectively by 3,6,9 W/W drug/material ratio. Then making the mixed material into standard pieces for test the adherence of candida albican on the surface by culture, counting the cloning. [Results] Among four kinds of materials, the pieces with Nystatin showed significantly stronger anti-fungal ability than the soft lining materials alone. The antifangal ability decreased within 6-8 days. No significantly difference observed between the 6%w/w and 9%w/w for the same material, however, both showed higher antifungal ability than samples with 3% Nystatin or with 0 % Nystatin. [Conclusion] For any soft liner material, the inhibition ability to candida albican is associated with the concentration of Nystatin added. The reasonable effect can be achieved by 6% w/w, and decreased by time. It suggests that Nystatin may be added into soft lining material to prevent denture stomatitis in short time purpose.