检测乙脑患儿血清和脑脊液中IL-18的临床意义

Clinical significance of interleukin-18 changes in serum and cerebrospinal fluid in children with epidemic encephalitis B

目的检测乙型脑炎患儿血清和脑脊液白细胞介素-18(IL-18)的水平,并探讨其临床意义。方法采用双抗夹心酶联免疫吸附实验检测32例乙脑患儿血清及脑脊液中IL-18含量。结果乙脑患儿极期血清及脑脊液中IL-18含量均明显高于对照组(P<0.01),差异有显著性。重型乙脑患儿血清及脑脊液中的IL-18含量明显高于普通型乙脑患儿(P<0.01),且恢复期血清中IL-18含量仍高于对照组(P<0.01)。普通型血清恢复期IL-18含量与对照组比较无显著差异性(P>0.05)。结论血清及脑脊液中IL-18含量增高与脑实质损伤程度密切相关,其含量增高可作为估计乙型脑炎病情及预后的一项重要指标,并对乙脑免疫调节方面的治疗具有一定的参考价值。

[Objective] To detect interleukin-18（IL-18） levels in children with epidemic encephalitis B and probe its clinical significance. [Methods] The IL-18 levels in serum and cerebrospinal fluid were measured in 32 patients with epidemic encephalitis B by a double-antibody sandwich ELISA. [Results] It was found that the level of IL-18 was significantly higher in fastigium phage of epidemic encephalitis B than that in control group （P〈0.01）. The average value of severe type in the patients with epidemic encephalitis B was significantly higher than that in common type （P〈0.01）, and level of IL-18 was still higher than that in control group in convalescenee （P〈0.01）. There was no significant difference in IL-18 level between the common type group and control group in convales-cence. [Conclusion] The results indicate that the level of IL-18 is elevated in serum and cerebrospinal fluid of epidemic encephalitis B and it is related to the degree of parenchymal lesions of brain. These researches suggest that the elevation of IL-18 may be an important marker of the parenchymal lesions of brain in patients with epidemic encephalitis B, and have some reference value in the immunoloregulation treatment for eoidemic encephalitis B.