大肠癌组织中survivin基因表达谱的改变

Expression abundance of survivin in colorectal carcinoma

目的研究大肠癌组织中凋亡抑制因子survivin表达水平的变化,探讨survivin在大肠癌发生中的作用。方法收集60例临床大肠癌和20例正常大肠组织标本,用半定量RT-PCR和western blot方法检测大肠癌及正常大肠组织中survivin的表达浓度,比较表达差异。结果大肠癌组织中survivin基因mRNA表达总阳性率为58.3%(35/60),显著高于正常大肠组织0%(0/25),P<0.01;Western blot验证结果表明大肠癌标本组织中survivin蛋白检测表达为阳性,其中正常大肠组织5例中仅有1例survivin微弱表达,进一步证实了survivin蛋白在大肠癌中表达增高。结论Survivin的过表达同大肠癌的发生相关。

[Objective] To investigate the expression of survivin in the colorectal carcinoma tissues. [Methods] RT-PCR and Western blot were used to detect the expression levels of survivin in 60 cases of colorectal carcinoma and 20 cases of normal colorectal tissues samples. [Results] The RT-PCR data of 60 cases of eolorectal carcinoma samples showed that the positive rate of survivin in tumor tissues was obviously higher than that in normal tissues （58.3% vs 0%, P〈0.01）. The Western blot results demonstrated that the survivin protein expression of colorectal carcinoma tissues was detectable but not detected in normal tissues. The Western blot further confirmed that survivin expressed significance in coloreetal carcinoma. [Conclusion] Over expression of survivin must be involved in the colorectal carcinoma.