茵陈化学成分的分离与鉴定

Isolation and identification of chemical constituents from Artemisia capillaries Thunb.

目的研究茵陈的化学成分，为茵陈药材的质量控制提供一定的依据。方法采用硅胶柱色谱、羟丙基葡聚糖凝胶柱色谱、制备液相等方法进行分离，根据理化性质和^1H-NMR、^13C-NMR等谱学技术对分离得到的化合物进行结构鉴定。结果从茵陈中分离得到8个化合物，分别鉴定为绿原酸（chlorogenic acid，1）、咖啡酸（caffeic acid，2）、异槲皮苷（isoquercitrin，3）、异鼠李素-3-O-葡萄糖苷（isorhamnetin-3-O-glucoside，4）、6，7-二羟基香豆素（6，7-dihydroxyl-coumarin，5）、熊果酸（ursollc acid，6）、齐墩果酸（oleanolic acid，7）、β-谷甾醇（β-sitosterol，8）。结论化合物5～7为首次从茵陈中分离得到。

Objective To study the chemical constituents of Artemisia capillaries Thunb. Methods Compounds were isolated by the silica gel, Sephadex LH- 20 columns and the preparative HPLC, and their structure were identified by the physicochemical characters and the spectral analysis. Results Eight compounds were obtained and identified as chlorogenic acid（l）, caffeic acid（2）, isoquercitrin（3）, isorhamnetin-3- O-glucoside（4）, 6, 7-dihydroxyl-coumarin（5）, ursolic acid（6）, oleanolic acid（7） and β-sitosterol（8）. Conclusions Compounds 5, 6 and 7 are obtained from Artemisia capillaries Thunb. for the first time.