射干化学成分的分离与鉴定

Isolation and identification of chemical constituents from the rhizome of Belamcanda chinensis(L.) DC.

目的分离并鉴定射干(Belamcandachinensis(L.)DC.)中的化学成分,以寻求具有活性的化合物,为中药新药研发奠定基础。方法采用硅胶柱色谱、聚酰胺柱色谱、羟丙基葡聚糖凝胶柱色谱、重结晶等多种分离方法,对射干的乙醇提取物进行分离;根据理化性质和波谱学数据鉴定其化学结构。结果分离鉴定了9个化合物,分别为次野鸢尾黄素(irisfloretin,1)、β-胡萝卜苷(β-daucos-terol,2)、白射干素(dichotomitin,3)、野鸢尾黄素(irigenin,4)、鸢尾苷元(tectorigenin,5)、β-谷甾醇(β-sitosterol,6)、罗布麻宁(apocynin,7)、尿嘧啶核苷(uridine,8)、环阿尔廷醇(cycloartanol,9)。结论化合物8、9为首次从射干属植物中分离得到。

Objective To isolate the chemical constituents and elucidate their structures from the rhizome of Belamcanda chinensis （L.） DC. Methods The compounds from the ethanol extraction were isolated by silica gel, polyamide, Sephadex LH-20 and recrystallization, and their structures were identified by the spectral analysis and chemical evidence. Results Nine compounds were obtained and elucidated as： Irisfloretin （1）, β- daucosterol （2）, dichotomitin （3）, irigenin （ 4 ）, tectorigenin （ 5 ）, β-sitosterol （ 6 ）, apocynin （7）, uridine （8）, and cycloartanol （9）. Conclusions Compounds 8 and 9 are isolated from the Belamcanda for the first time.