HPLC法测定辽东楤木叶中木芽皂苷A的含量

Content determination of congmuyanoside A in leaves of Aralia elata(Miq.) Seem. by HPLC

目的建立辽东楤木叶中楤木芽皂苷A的含量测定方法，为辽东楤木叶的质量评价提供依据。方法采用HPLC法。色谱柱为ODS柱（150mm×4．6mm，5μm），流动相为乙腈-水-磷酸（体积比35：65：0．2）。流速为0．8mL·min^-1，检测波长为206nm，柱温为28℃。结果楤木芽皂苷A质量在0.6～4．0μg内与峰面积呈良好的线性关系，其回归方程为A=3．941×10^5 m＋4．142×10^2，r=0．9997（n=5），平均回收率为99．6％，RSD为2．9％（n=9）。结论采用HPLC法测定辽东楤木叶中楤木芽皂苷A的含量，结果准确、可靠；该法可用于辽东楤木叶的质量控制。

Objective To develop an HPLC method for determination of congmuyanoside A in leaves of Aralia elata （Miq.）Seem., and provide the basis for quality specification of leaves of Aralia elata （Miq.） Seem. Methods Chromatography was carried out on ODS column（ 150 mm×4.6 mm, 5μm）, using acetonitrile-H2O-H3PO4（ V：V：V = 35：65：0.2） as the mobile phase at a flow rate of 0.8 mL·min^-1. The UV detection wavelength was set at 206 nm. The temperature of the column was 28 ℃. Results The linear range of congmuyanoside A was 0.6 - 4.0 μg（ r = 0. 999 7）and the standard curve was： A = 3.941×10^5 m ＋ 4.142×10^2. The average recovery of congmuyanoside A was 99.6 % （RSD = 2.9 %, n = 9）. Conclusions This method can be used to determinate congmuyanoside A in leaves of Aralia elata （Miq.）Seem. and provides the basis for quality specification of leaves of Aralia elata （Miq.）Seem.