HPLC法同时测定痔痛安搽剂中三种蒽醌类成分的含量

Content determination of emodin,chrysophanol and physcion in Zhitong'an liniment by HPLC

目的建立同时测定痔痛安搽剂中三种蒽醌类成分大黄素、大黄酚和大黄素甲醚含量的方法，提高制剂的质量控制标准。方法采用HPLC法。色谱柱为Diamonsil C18柱，流动相为甲醇-质量分数为0．1％的磷酸水溶液（体积比75：25），流速1．0mL·min^-1，检测波长254nm，柱温35℃。结果大黄素、大黄酚、大黄素甲醚质量浓度分别在0．64～20．48mg·L^-1（r=0．9995）、0．31～9．84mg·L^-1（r=0．9997）、0．50～16．08mg·L^-1（r=0．9998）内与峰面积呈良好的线性关系；大黄素平均加样回收率为98．8％，大黄酚平均加样回收率为99．6％，大黄素甲醚平均加样回收率为98．5％。结论采用HPLC法可同时测定痔痛安搽剂中三种蒽醌类成分大黄素、大黄酚和大黄素甲醚的含量，且操作方法简便，分离效果好，运行成本低。该法可用于痔痛安搽剂的质量评价。

Objective To develop a method for determination of emodin, ehrysophanol and physeion in Zhitong＇ an（traditional Chinese medicines） liniment. Methods RP-HPLC method was used with Diamonsil C18 （250 mm×4.6 mm, 5 μm） column. The mobile phase was methanol-0.1% phosphoric acid （ V： V= 75：25）, flow rate was 1.0 mL·min^-1. The detection wavelength was set at 254 nm, and column temperature was 35 ℃. Results The calibration curves were linearin the range of 0.64- 20.48 mg·L^-1 （r = 0.999 5）for emodin, 0.31-9.84 mg·L^-1（r =0.999 7） for ehrysophanol and 0.50-16.08 mg·L^-1（r= 0. 999 8） for physeion. The average recoveries of emodin, physeion and ehrysophanol were 98.8 %, 99.6 % and 98.5%, respectively. Conclusions The method is convenient and appropriate for determination of emodin, ehrysophanol and physeion in Zhitong＇ an liniment.