奶牛子宫内膜炎病原菌的分离鉴定及ε-多聚赖氨酸的抑菌作用

Isolation and Identification of Pathogenic Bacteria from Endometritis in Dairy Cows and Bacteriostasis of ε-polylysine

为了研究ε-多聚赖氨酸对奶牛子宫内膜炎病原菌的抑菌作用,采集杭州地区患牛子宫内膜分泌物病料26份进行细菌分离鉴定及药敏试验,并采用试管稀释法测定了ε-多聚赖氨酸对分离到的病原菌的最小抑菌浓度。结果,从26头子宫内膜炎患牛的子宫分泌物中检测到21份分泌物中有细菌,分别是凝固酶阴性葡萄球菌(19.2%,5/26)、牛链球菌(15.4%,4/26)、化脓链球菌(7.7%,2/26)、大肠杆菌(15.4%,4/26)、普通变形杆菌(30.8%,8/26)、肺炎克雷伯菌(11.5%,3/26);5份分泌物中未检测到细菌(19.2%,5/26)。药敏试验结果表明,多数病原菌对多西环素和苯唑青霉素耐药,但对环丙沙星、庆大霉素、氧氟沙星敏感。试管稀释法结果表明,ε-多聚赖氨酸对子宫分离菌的最小抑菌浓度(MIC)为45～215μg/mL。由于ε-多聚赖氨酸对奶牛子宫内膜炎致病菌具有良好的抑菌作用以及在食品中的安全性,有必要进一步研究其对奶牛子宫内膜炎的治疗作用。

To investigate the bacteriostasis of ε - polylysine to bovine endometritis pathogenic bacteria, 26 uterus secretory samples were collected from the cows with endometritis on dairy farms in Hangzhou and the pathogenic bacteria were isolated and identified, and the minimal inhibitory concentrations of ε - polylysine were determined for these pathogenic bacteria by the tube dilution method. The results indicated that twenty - one bacterial isolates were obtained from the 26 uterus secretions of dairy cows with endometritis. They were coagulase negative Staphylococci （ 19.2%, 5/26）, Streptococcus boris（ 15.4% ,4/26）, Streptococcus pyogenes（ 7. 7% ,2/26）, Escherichia coli（ 15.4% ,4/26）, Proteus vulgaris （ 30.8%, 8/26 ） and Klebsiella pnenmoniae （ 11.5%, 3/26 ）, respectively. Five samples had no bacteria detected（ 19.2% ,5/26）. These isolates were resistant to doxycycline and oxacillin, but sensitive to ciprofloxacin, gentamicin and ofloxacin. The test tube dilution method demonstrated that the minimum inhibitory concentration of ε - Polylysine to the bacterial isolates were 45 - 215 μg/mL. ε - polylysine should be further evaluated for its therapeutic effect on endometritis in dairy cows due to its strong inhibitory effect on the endometritis- causing bacteria and safety in food.