升清胶囊下调胆囊结石豚鼠胆囊上皮组织雌、孕激素受体

Shengqing Capsule down-regulates estrogen and progesterone receptors in epithelial tissue of gallbladder in guinea pigs with gallstone

目的:研究雌激素受体和孕激素受体在胆固醇结石形成中的作用及升清胶囊对其表达的影响。方法:将80只雌性豚鼠随机分为正常对照组、模型组、熊去氧胆酸组和升清胶囊组,每组20只。除正常对照组外,采用高脂致石饮食法建立胆囊胆固醇结石模型。升清胶囊组和熊去氧胆酸组豚鼠分别灌胃相应药物。造模7周后观察各组豚鼠胆囊成石情况,并采用免疫组织化学法检测豚鼠胆囊上皮组织雌、孕激素受体的表达。结果:升清胶囊可降低造模豚鼠的成石率,与模型组比较,差异有统计学意义(P<0.05)。按正常对照组、升清胶囊组、熊去氧胆酸组和模型组的顺序,各组豚鼠胆囊组织雌、孕激素受体阳性表达半定量分级逐级升高,升清胶囊组与模型组、熊去氧胆酸组比较差异有统计学意义(P<0.05)。各组间雌、孕激素受体阳性表达率比较,差异有统计学意义(P<0.05)。结论:疏肝利胆方药升清胶囊可下调雌、孕激素受体的表达,降低胆固醇结石形成。

Objective： To study the role of estrogen receptor（ER） and progesterone receptor（PR） in the formation of cholesterol calculus and investigate the effects of Shengqing Capsule（SQC）,a Chinese patent herbal medicine with the function of soothing liver and draining gallbladder,on ER and PR expressions.Methods： A total of 80 female guinea pigs were divided into normal control group,untreated group,ursodeoxycholic acid group（UDCA group） and SQC group.The cholesterol gallstone was induced by feeding the guinea pigs with high-fat lithogenic diet.SQC and UDCA were separately administered to the guinea pigs in the SQC group and UDCA group.After 7-week administration,all the animals were sacrificed to calculate the incidence of calculus formation and detect the expressions the ER and PR in the epithelial tissue of gallbladder by immunohistochemical method.Results： Gallstone was cholesterol calculus detected by infrared spectrum.The incidence of calculus formation in the SQC group（27.78%） was significantly lower than that in the untreated group（81.25%）（χ^2=9.721 5,P=0.001 8）.On the basis of Reiner standard,the expression distribution of ER and PR increased gradually from the normal control group through the SQC group and UDCA group to the untreated group.Except for the former two groups and the latter two groups,the differences between the other groups and UDCA group were statistically significant（P〈0.05）.Besides,the differences of positive expression rates between groups were statistically significant（P〈0.05）.Conclusion： Increased expressions of ER and PR are closely related to the formation of cholesterol stone.And Shengqing Capsule can down-regulate the expressions of ER and PR.