补益气血方剂对大鼠慢性难愈性创面血管内皮生长因子及血管生成的影响

Effects of reinforcing qi and nourishing blood recipes on vascular endothelial growth factor and microvessel count in granulation tissue in rats with chronic skin ulcers

目的:探讨补益气血方剂四君子汤、四物汤和八珍汤对慢性难愈性创面大鼠创面血管内皮生长因子(vascular endothelial growth factor,VEGF)及微血管数(microvessel count,MVC)的影响。方法:在90只雄性SD大鼠背部制造全层皮肤缺损开放性创面。除正常对照组外,大鼠肌肉注射醋酸氢化可的松建立难愈性创面模型,随机分为模型组、四君子汤组、四物汤组和八珍汤组。观察创面愈合时间;通过免疫组化结合图像分析等技术,观察各组大鼠创面肉芽组织中VEGF表达情况和MVC。结果:模型组创面愈合时间较正常组明显延长(P<0.01),各治疗组创面愈合时间比模型组缩短(P<0.01,P<0.05),四物汤组和八珍汤组创面愈合时间较四君子汤组延长(P<0.05)。模型组VEGF表达和MVC明显低于正常对照组(P<0.01),各治疗组VEGF表达和MVC高于模型对照组(P<0.01,P<0.05),低于正常对照组(P<0.01)。四物汤组和八珍汤组VEGF表达和MVC低于四君子汤组(P<0.05,P<0.01),四物汤组和八珍汤组比较,差异无统计学意义(P>0.05)。结论:补益气血方剂能明显促进慢性难愈性创面大鼠的创面愈合,其机制可能与上调VEGF表达水平,诱导血管新生有关。

Objective： To explore the effects of reinforcing qi and nourishing blood recipes on vascular endothelial growth factor（VEGF） and the microvessel count（MVC）in granulation tissue in rats with chronic skin ulcers.Methods： A total of 90 male SD rats with back full-thickness skin lesion were used in this study.Except for the normal control group,the rats were injected with hydrocortisone for inducing chronic skin ulcers,and were randomly divided into untreated group,Sijunzi Decoction group,Siwu Decoction group and Bazhen Decoction group.The time of wound healing was observed, immunohistochemical method and image analytical method were used to test the expressions of VEGF and MVC in granulation tissue of dermal chronic ulcers in the rats.Results： In the untreated group,the time of wound healing was significantly longer than that in the normal group（P〈0.01）.The time of wound healing was obviously shortened in the three treated groups as compared with the untreated group（P〈0.01,P〈0.05）.In the Sijunzi Decoction group,the time of wound healing was obviously shorter than those in Siwu Decoction group and Bazhen Decoction group（P〈0.05,P〈0.01）.In the untreated group,the expression of VEGF and MVC were significantly lower than those in the normal control group（P〈0.01）.The expression of VEGF and MVC were obviously increased in the three treated groups as compared with those in the untreated group（P〈0.01,P〈0.05）.In the Sijunzi Decoction group,the expression of VEGF and MVC were obviously lower than those in Siwu Decoction group and Bazhen Decoction group（P〈0.05,P〈0.01）.Conclusion： Reinforcing qi and nourishing blood recipes can promote wound healing in rats through up-regulating the expression of VEGF in granulation tissue of dermal chronic ulcers,and inducing angiogenesis.