柴芩承气汤减轻急性胰腺炎大鼠胰腺钙超载的机制研究

Chaiqin Chengqi Decoction decreases pancreatic acinar cell calcium overload in rats with acute pancreatitis

目的:观察柴芩承气汤(Chaiqinchengqi decoction,CQCQD)对急性胰腺炎大鼠胰腺组织肌浆网钙ATP酶(sarcoendoplasmic reticulum Ca2+-ATPase,SERCA)mRNA表达的影响。方法:30只SD大鼠随机分成正常对照组、模型组及CQCQD组。采用蛙皮素腹腔注射法建立急性胰腺炎大鼠模型。逆转录聚合酶链式反应检测胰腺组织SERCA1和SERCA2 mRNA表达的变化;激光共聚焦显微镜检测腺泡细胞内钙离子浓度,并比较各组大鼠胰腺组织病理变化。结果:SERCA1 mRNA在正常胰腺和急性胰腺炎胰腺腺泡细胞均不表达。模型组与正常对照组比较,腺泡细胞SERCA2 mRNA表达下调,表达率为0.536(P=0.001);CQCQD组与模型组比较,腺泡细胞SERCA2 mRNA的表达上调,表达率为1.803(P=0.035)。模型组大鼠细胞内钙离子的荧光强度高于正常对照组及CQCQD组(P<0.05);CQCQD组大鼠胰腺组织的病理评分低于模型组(P<0.05)。结论:CQCQD可以通过上调SERCA2 mRNA表达,减缓胰腺细胞内钙超载,减轻胰腺组织的病理改变。

Objective： To investigate the mechanism of Chaiqin Chengqi Decoction（CQCQD）,a compound of traditional Chinese herbal medicine,acting on the pancreatic acinar cell calcium overload in rats with acute pancreatitis（AP）.Methods： A total of 30 SD rats were randomly divided into normal control group,untreated group and CQCQD group（n=10,respectively）.AP was induced in rats by caerulein（5×50 μg/kg） intraperitoneal injection within 4 h.The pancreatic tissue SERCA1 and SERCA2 mRNA expressions were detected by fluorescent quantization polymerase chain reaction method, intracellular calcium fluorescence intensity（FI） of pancreatic acinar cells and the pancreatic pathological score were measured by laser scanning confocal microscopy and light microscopy respectively.Results： There were no SERCA1 mRNA expressions in pancreatic acinar cells of rats in the normal control group and the untreated group.The expression of pancreatic SERCA2 mRNA in the untreated group was down-regulated compared with that in the normal control group（expression ratio=0.536, P=0.001）, the expression of pancreatic SERCA2 mRNA in the CQCQD group was up-regulated compared with that in the untreated group（expression ratio=2.00, P=0.012）.The pancreatic pathological score in the CQCQD group was lower than that in the untreated group and the FI of Ca^2＋ was also lower.Conclusion： CQCQD can up-regulate the expression of pancreatic SERCA2 mRNA,release the calcium overload,and hence reduce the pathological changes in pancreatic tissue.