缺氧对神经胶质细胞hif-1基因表达和凋亡的影响

Effects of hypoxia on expression of hif-1 gene and apoptosis of gliacytes

目的探讨缺氧环境对培养的新生鼠大脑胶质细胞低氧诱导因子-1(HIF-1)基因表达和凋亡的影响。方法体外培养SD新生鼠大脑神经胶质细胞;实验分为对照组:正常培养6h(5%CO2、95%空气)和缺氧组:缺氧培养6 h(5%CO2、1%O2、94%N2)。实时定量多聚酶链反应(PCR)方法测定细胞hif-1和bcl-2、bax基因表达。结果缺氧组hif-1 mRNA表达高于对照组(P<0.01);缺氧组bcl-2 mRNA表达低于对照组(P<0.05),bax mRNA表达高于对照组(P<0.01)。结果缺氧6 h可引起胶质细胞hif mRNA表达增加但促进细胞凋亡。

Objective To investigate the effects of hypoxia condition on gliacytes apoptosis and expression of hypoxia inducible factor-1 （HIF-1）. Methods The cultured gliacytes were randomly divided into two groups： control group （cultivated 6 h without hypoxia） and hypoxia group （cultivated 6 h under hypoxia） and then the ceils were collected. The expressions of hif-1, bcl-2 and bax gene were detected by real time polymerase chain reaction （PCR）. Results hif-1 expression of hypoxia group increased more obviously （P 〈 0. 01 ） than that of control group ; bcl-2 content of hypoxia group was lower than that of control group （ P 〈0.05 ） ; expression of bax content was higher in hypoxia group than that in control group （P 〈 0. 01 ）. Conclusion Hypoxia may induce the increased expression of hif-Ⅰ and gliacytes apoptosis.