鹅Myostatin基因单核苷酸多态性检测及群体遗传分析

Identification and Genetic Analyses of Single Nucleotide Polymorphisms in Goose Myostatin Gene

肌肉生长抑制素是TGF-β超家族的新成员,其主要在骨骼肌中特异性表达,且对骨骼肌的生长发育起负调控作用。本研究采用PCR-SSCP和DNA测序的方法检测了鹅Myostatin基因的单核苷酸多态性,并对浙东白鹅、五龙鹅、扬州鹅、朗德鹅、莱茵鹅、豁眼鹅、吉林农大白鹅、狮头鹅、皖西白鹅、籽鹅等不同品种(系)鹅单核苷酸多态性进行了群体遗传分析。结果表明:(1)在检验的12对Myostatin基因的引物中,发现3对引物的扩增片段具有多态性,分别为启动子区域T769G、C543T的突变,内含子1的A1 632G突变。(2)不同鹅品种(系)群体遗传学分析表明,启动子区域引物P3扩增片段多态性位点在各群体中等位基因A的频率均高于B,且等位基因频率均高于0.77,表现为优势等位基因;引物P4扩增片段多态性位点在扬州鹅、朗德鹅、狮头鹅群体中等位基因D的频率均高于C的频率,等位基因D的频率分别为0.625 0、0.548 8、0.647 4,而在其余群体中均表现为等位基因C的频率高于D的频率。

Myostatin, a new member of the TGF-β super family, is specifically expressed in skeletal muscle cells and functions as a negative regulator of skeletal muscle growth. In this study, the single nucleotide polymorphisms （SNPs） of Myostatin gene was identified in various goose breeds including Zhedong White geese, Wulong geese, Yangzhou geese, Landish geese, Rheine geese, Huo geese, Jilin White geese, Shitou geese, Wanxi White geese, Zi geese, etc. by PCR-SSCP and DNA sequencing. The results showed that： （1） The 12 pairs of primers for goose Myostatin gene were designed based on goose Myostatin gene sequence. Three SNPs located in the promoter region of the Myostatin （T769G, C543T ） and the intron 1 （A1 632G） were identified in geese Myostatin gene, respectively. （2）Population genetic analyses indicated that frequency of allele A （ P3 ） in promoter region was over 0. 77 and higher than that of allele B, which is a dominant allele. Frequency of allele D （ P4 ） among Yangzhou geese, Landish geese, and Shitou geese were higher than that of allele C, and the frequency of allele D were 0. 625 0, 0. 548 8 and 0. 647 4 among the three breeds, respectively. But on the contrary, it presented that frequency of allele C is higher than that of allele D in other breeds.