摘要
目的建立类风湿关节炎(RA)患者骨髓间充质干细胞(BMSCs)的体外培养、扩增方法,了解其一般生物学特性。方法采集RA患者的骨髓标本,经密度梯度离心加贴壁法分离、纯化获得其BMSCs,进行体外培养扩增,观察原代和传代细胞的形态和生长状况、免疫表型分析及增殖能力检测,并对BMSCs进行成纤维细胞集落(CFU—F)形成分析。结果来自RA患者的BMSCs呈典型的成纤维样细胞形态,表型鉴定CD45阴性,SH2(CD105)、CD71、CD44阳性,其增殖能力和CFU—F形成能力与源于正常供者的BMSCs相当。结论RA患者骨髓中存在具有多向分化能力的MSCs,来源于RA患者的BMSCs在体外具有良好的细胞增殖能力,在细胞形态、集落形成能力以及细胞免疫表型方面与正常供者的BMSCs差异无统计学意义。
Objective To isolate and culture bone marrow mesenchymal stem cells (BMSCs) from patients with rheumatoid arthritis (RA) and examine their biological characteristics. Methods MSCs were isolated from bone marrow of RA patients and purified by density gradient centrifugation and cultured in vitro. The morphology, immunophenotype, and proliferative property of BMSC and colony forming unit-fibroblast (CFU-F) were measured and analyzed. Results The culture expanded cells from RA patients presented a typical fibroblast-like morphology. Ceils were positive for SH2 (CD105), CD71, and CD44, but negative for CD45. Their proliferative capacity and CFU-F number were similar to those of BMSCs from healthy donors. Conclusion In respect to morphology, immuno-phenotype, proliferative property and colony forming unitfibroblast (CFU-F),MSCs from bone marrow of RA patients are not different from those of MSCs isolated from bone marrow of normal donors. MSCs from the bone marrow of RA patients have the potential for clinical application.
出处
《中华风湿病学杂志》
CAS
CSCD
2008年第10期680-683,730,共5页
Chinese Journal of Rheumatology