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G-CSF动员的外周血VoL24^＋NKT细胞体外扩增及免疫表型

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摘要 NKT细胞识别由抗原呈递细胞（APC）表面CD1d分子提呈的脂类抗原，人工合成的α-半乳糖酰基鞘胺醇（α—galactosylceramide，α—GalCer，KRN7000）可以激活NKT细胞^[1]。NKT细胞在体内活化后同时分泌Th1和Th2型细胞因子^[2]，不同条件下的活化可导致Th1或Th2方向的免疫反应。我们早期研究发现成人外周血或脐血中的Vα24^＋NKT细胞可在含α-GalCer、IL-2和IL-15的短期培养体系中大量扩增^[3]。
作者 翟卫华 黄勇 王玫 周征 翟文静 张荣莉 王华 王志永 包宇实 杜宏 韩明哲
机构地区 中国医学科学院、北京协和医学院血液学研究所、血液病医院、实验血液学国家重点实验室
出处 《中华血液学杂志》 CAS CSCD 北大核心 2008年第10期708-709,共2页 Chinese Journal of Hematology
关键词 成人外周血 G-CSF动员 细胞体外扩增 免疫表型 Th2型细胞因子 NKT细胞 Α-GALCER CD1d分子
分类号 R686 [医药卫生—骨科学]
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参考文献10

  • 1Kawano T, Cui J, Koezuka Y, et al. CDI d-restricted and TCR-mediated activation of Valpha14 NKT Cells by glycosylceramides. Science, 1997,278 : 1626-1629.
  • 2Godfrey DI, Hammond KJ, Poulton LD, et al. NKT cells:facts,functions and fallacies. Immunol Today,2000,21:573-583.
  • 3黄勇,姜尔烈,周征,何祎,王玫,刘庆国,翟文静,韩明哲.经α-半乳糖酰基鞘氨醇体外扩增Vα24自然杀伤T细胞的生物学特性[J].中国医学科学院学报,2005,27(3):315-320. 被引量：2
  • 4Imataki O, Heike Y, lshida T, et al. Efficient ex vivo expansion of Vα24^+ NKT cells derived from G-CSF-mobilized blood cells. J Immunother, 2006,29 : 320 -327.
  • 5Yu KO, Im JS, Molano A, et al. Modulation of CDld-restricted NKT cell responses by using N-acyl variants of alpha-galactosylceramides. Proc Natl Acad Sci U S A,2005,102:3383-3388.
  • 6Sonoda KH, Nakamura T, Young HA, et al. NKT cell-derived urokinase-type plasminogen activator promotes peripheral tolerance associated with eye. J Immunol, 2007, 179:2215-2222.
  • 7Yang SH, Jin JZ, Lee SH, et al. Role of NKT cells in allogeneic islet graft survival. Clin Immunol, 2007,124:258-266.
  • 8Vela-Ojeda J, Garcia-Ruiz Esparza MA, Reyes-Maldonado E,et al. Clinical relevance of NK,NKT,and dendritic cell dose in patients receiving G-CSF-mobilized peripheral blood allogeneic stem cell transplantation. Ann Hematol,2006,85 : 113-120.
  • 9Pillai AB, George TI, Dutt S, et al. Host NKT cells can prevent graft-versus-host disease and permit graft antitumor activity after bone marrow transplantation. J Immunol,2007,178:6242-6251.
  • 10Haraguchi K, Takahashi T, Hiruma K, et al. Recovery of Valpha 24 + NKT cells after hematopoietic stem cell transplantation. Bone Marrow Transplant,2004,34:595- 602.

二级参考文献8

  • 1Wilson MT, Johansson C, Olivares-Villagomez D, et al.The response of natural killer T cells to glyeolipid antigens is characterized by surface receptor down-modulation and expansion. Proc Nati Aca Sci USA, 2003, 100:10913-10918
  • 2Seino K, Fukao K, Muramoto K, et al. Requirement for natural killer T (NKT) cells in the induction of allograft tolerance. Proc Natl Acad Sci USA, 2001, 98:2577-2581
  • 3Baker J, Vemeris MR, Ito M, et al. Expansion of eytolytie CD8+ natural killer T cells with limited capacity for graftversus-host disease induction due to interferon-γ production.Blood, 2001, 97:2923-2931
  • 4Fandrich F, Lin XB, Chai GX, et al. Preimplantation-stage stem cells induce long-term allogeneic graft acceptance without supplementary host conditioning. Nat Med, 2002,8171-178
  • 5Parham P, McQueen KL. Alloreactive killer cells: hindrance and help for haematopoietic transplants. Nat Rev Immunol,2003, 3:108-122
  • 6Godfrey DI, Hammond KJ, Poulton LD, et al. NKT cells:facts, functions and fallacies. Immunol Today, 2000, 21:573 -583
  • 7Kawano T, Cui J, Koezuka Y, et al. CD 1d-restricted and TCR-mediated activation of Vo14 NKT cells by glycosylceramides. Science, 1997, 278:1626-1629
  • 8Ranson T, Vosshenrich CAJ, Corcuff E, et al. IL-15 availability conditions homeostasis of peripheral natural killer T cells. Proc Nati Acad Sci USA, 2003, 100:2663-2668

共引文献1

中华血液学杂志
2008年 第10期

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