人核迁移蛋C刺激人造血干细胞增殖分化为巨核细胞研究

hNUDC promotes proliferation and differentiation of megakaryocytopoiesis on human CD34^+ cells

目的:研究人核迁移蛋C(hNUDC)促进人脐血来源的CD34+细胞增殖、分化为巨核细胞的作用。方法:使用DynalCD34体外分离系统收集人脐血CD34+细胞,无血清甲基纤维素半固体法体外培养CD34+细胞12d后,显微镜下观察hNUDC对CD34+细胞分化增殖为小、中、大巨核细胞集落的形态、数目的影响;无血清液体培养体系培养CD34+细胞10d后,流式细胞术检测hNUDC对CD34+细胞分化增殖为CD41+细胞的表达率及其DNA倍性的影响。结果:hNUDC能够明显促进CD34+细胞分化增殖形成中小型CFU-MK集落,可显著增加巨核细胞表面标志物CD41+的表达,CD41+细胞中DNA倍性显著地高于血小板生成素。结论:hNUDC对促进造血干细胞增殖和分化为巨核细胞具有重要作用。

AIM：To study the effect of human nuclear distribution C（hNUDC）on human megakaryocyte proliferation and differentiation from cord blood CD34＋ cells in vitro.METHODS：Human CD34＋ cells were isolated using the Dynal CD34 Progenitor Cell Selection System from umbilical cord blood.The CD34＋ cells were then cultured in serum free methylcellulose semi-solid media,the morphologic aspects and number of small,medium and large CFU-MK colonies were observed and scored on the day12 by microscopy analysis.The CD34＋ cells were cultured in serum free liquid media,cells were removed on day 10 and formation of CD41＋ in human megakaryocyte and its DNA polyploidization of nuclear were analyzed on a FACsort flowcytometer.RESULTS：hNUDC supported the formation of small and medium CFU-MK colony in serum free semi-solid media.Furthermore,hNUDC induced a remarkable increase in expression of the megakaryocyte cell surface marker CD41＋ and stimulated the CD41＋ DNA polyploidization more effectively than TPO.CONCLUSION：hNUDC may play an important role in megakaryocyte proliferation and differentiation.