重复加热致肝癌HepG2细胞对化疗药耐受性与细胞凋亡的变化

The change of apoptosis and chemotherapeutics resistance after multiple thermotherapy on the HepG2 cell

目的探讨多次加热肝癌HepG2细胞后，残存细胞对化疗药耐受性与细胞凋亡变化的关系。方法HepG2细胞43℃加热，每次80min，每天2次，10个循环后，MTT法分析其对阿霉素的敏感性，试剂盒检测细胞的凋亡，免疫组化法检测细胞Bcl-2蛋白及Bax蛋白的表达，RT-PCR法检测细胞Bcl-2mRNA及BaxmRNA的表达，结果热处理后的HepG2细胞对阿霉素的化疗耐受性升高，细胞凋亡率减低，Bcl-2基因／蛋白的表达增强，Bcl-2／Bax值增大。结论热处理后HepG2细胞对化疗药物耐受性增高可能与其凋亡率降低、Bcl-2基因／蛋白的表达增强及Bcl-2／Bax值增大有关。

Objective To investigate the change of apoptosis and drug resistance after multiple thermotherapy on the HepG2 Cell. Methods After HepC2 cell were treaded by ten repeated cycles of exposure at 43 degree C for 80 minutes twice a day, the sensibilily of HepC2 cell to Adriamycin was analyzed by MTT assay. The apoptosis was detected by kit, and the expression of Bcl-2 or Bax protein and mRNA were detected by immunohistochemical method and RT-PCR respective. Results The drug resistance to Adriamycin was gained by HepC2 cell after multiple thermotherapy. The rate of apoptosis was decreased, the expression of Bel - 2 protein and mRNA was strengthened, and the ratio of Bcl-2/Bax increased. Conclusions The resistance of heated HepG2 cell to chemotherapeuties was related with the decreased rate of apoptosis, overexpression of Bcl-2 protein and mRNA, and increased ratio of Bcl-2/Bax .