双歧杆菌对严重烧伤大鼠肠黏膜细胞增殖的影响

Effects of bifidobacterial supplement on expression of PCNA in intestinal mucosal of rats after severe burns

目的观察严重烫伤后补充外源性双歧杆菌对肠黏膜细胞增殖的影响。方法70只wistar大鼠随机分为烫伤并应用双歧杆菌组（A组），烫伤不应用双歧杆菌组（B组）和正常对照组（C组）。采用免疫组化SABC法，检测PCNA表达情况，反映肠黏膜细胞增殖情况。结果烫伤后A组和B组肠腺细胞PCNA表达减少；在烫伤后48h、72h、120h，A组PCNA表达较B组明显增多（P〈0．05）结论双歧杆菌可能促进严重烫伤大鼠肠腺细胞增殖，有利于损伤的肠黏膜修复。

Objective To study the potential effects of bifidobacterial supplement on expression of PCNA in intestinal mucosal of rats after severe burns. Methods Seventy Wistar rats were randomly divided into burn plus treatment group （ group A, n = 30） ,burn group （group B , n =30）, and control group （group C, n = 10）. The PCNA expression in intestinal mucosal were determined by immunohisto- chemical staining. Results The PCNA expression was decreased after thermal injury. The number of PCNA expressed cells in group B was signifieantly lower than that of group A. （ P 〈 0. 05）. Conclusion The supplement of exogenous bifidobaeterium can facilitate resuming of mucosal barrier function.