还原型谷胱甘肽对大鼠肝移植中胆管细胞缺血再灌注损伤的保护作用

The protection of Atomolan on lschemia-reperfusion injury of the rat bile duct cells in liver transplantation

目的探讨胆管上皮细胞缺血再灌注损伤的机制及阿拓莫兰在大鼠肝移植中胆管细胞I／R损伤中的作用。方法采用重建肝动脉血供的大鼠原位肝移植模型。分3组观察在不同条件下胆管细胞的病理形态变化及阿拓莫兰对此缺血再灌注损伤的影响。结果缺血再灌注组的胆管细胞损伤更重，血清ALT、AST水平，γ-谷氨酰转肽酶更高，氧自由基生成更多，胆管上皮细胞凋亡发生更多，阿拓莫兰预处理保护组胆管细胞损伤明显减轻。结论缺血再灌注损伤对胆管细胞的损伤更重，阿拓莫兰预处理可减轻该损伤，其机制可能为抑制氧自由基生成，减少胆管上皮细胞凋亡。

Objective To explore the mechanisms of ischemia-reperfusion （I/R） injury of the bile duct and the effect of Atomolan on lschemia-reperfusion injury of the bile duct in liver transplantation. Methods Pathological changes of the bile duct cells in three groups of rat liver transplantation model were observed through light microscope. And the effective protection of Atomolan against ischemia-reperfusion injury was observed. Results In ischemia - reperfusion group, the injury of bite duct cells were more serious, the level of ALT, AST and γ-glutamyltransferase elevated higher, oxygen free radical generated more, and the apoptosis of bile duct cells appeared more, compared with Atomolan pretreatment . Conclusion The injury in bile duct cells is more serious than that in hepatocyte in I/R injury. Atomolan can provide effective protection against liver Ischemia-reperfusion injury. The mechanisms may be through depress the production of oxygen free radical and decrease the apoptosis of bile duct cells.