切花月季开花进程差异与花瓣内肽酶活性的关系

Relationship Between Endopeptidase Activity in Petals and the Process of Opening to Senescence of Two Cut Rose Cultivars

以瓶插期开花进程不同的切花月季萨蔓莎和贝拉米为试材,探讨了切花月季开花进程差异与花瓣内肽酶活性的关系。结果表明:开花进程中萨蔓莎花瓣起主导作用的是pH 10下的内肽酶,贝拉米花瓣中起主导作用的是pH 6下的内肽酶,且前者活性远高于后者的。开花进程中花瓣金属蛋白酶在萨蔓莎中一直存在且活性较高,而在贝拉米中逐步被启动,初开期表现活性且随开花进程而增大;花瓣巯基蛋白酶在萨蔓莎盛开之前存在,之后活性消失,而在贝拉米盛开之后活性升高;花瓣酸性丝氨酸蛋白酶在二者中活性均基本不变,但整体上在萨蔓莎中的活性高于贝拉米的;花瓣碱性丝氨酸蛋白酶在盛开期前萨蔓莎中的活性远高于贝拉米的,盛开后相反。由此推测,初开期金属蛋白酶的诱导差异及盛开前后巯基蛋白酶和碱性丝氨酸蛋白酶的活性差异可能与二者开花进程差异有关。

This work was aimed the investigate to relationship between the difference in the process of opening to senescence and the activity and type of endopeptidase（EP） in different cultivars of cut roses（Rosa hybrid）.Two cultivars,＇Samantha＇ and ＇Belami＇ were used as materials.In the process of opening to senescence,the former was faster than the later,and the former can open enough largly,while the later becomes always stereotyped.The results showed that,In the process of opening to senescence,EP activity of pH 10 played a key role in petals of ＇Samantha＇ and EP activity of pH 6 played a key role in petals of ＇Belami＇,at the same time,EP activity of the former was higher by far than that of the later.In the process of opening to senescence,activity of metallo-proteinase in ＇Samantha＇ petals was always high,while that in ＇Belami＇ petals was activited at the stage 3 and increased with the process of floral opening.Activity of thiol-proteinase in ＇Samantha＇ petals was higher before the stage 4,that was out after the stage 4,while that in ＇Belami＇ petals increased after stage 4.Acidic serine proteinase of ＇Samantha＇ and ＇Belami＇ petals all kept invariability,but activity of ＇Samantha＇ petals was higher than that of ＇Belami＇ petals.Activity of alkaline serine proteinase in ＇Samantha＇ petals was higher by far than that in ＇Belami＇ petals before the stage 4,the station was oppositive after the stage 4.The results above suggested that the difference of the process of opening to senescence between two cultivars might be related to the induced difference of metallo-proteinase at the stage 3 and the difference of thiol-proteinase and seriene proteinase activities in petals before and after the stage 4.