莪术醇诱导白血病L1210细胞凋亡作用研究

L1210 Cell Apoptosis Induced by Curcumenol

目的:探讨莪术醇体外抗白血病的作用及机制,为开发利用莪术醇提供理论依据。方法:采用机械分散法结合超声波法制备莪术醇脂质体。以MTT法测定莪术醇等对小鼠淋巴细胞白血病L1210细胞生长的抑制作用。用台盼蓝拒染法及Hoechst 33258/PI双染色,激光共聚焦显微镜观察莪术醇诱导白血病L1210细胞凋亡的作用。结果:莪术醇能显著抑制白血病L1210细胞生长,诱导白血病L1210细胞凋亡。结论:莪术醇诱导肿瘤细胞凋亡是其抗肿瘤作用的重要机制之一。

OBJECTIVE： To investigate the effect and mechanism of Curcumenol against leukemia and to provide theoretical basis for the development and utilization of Curcumenol. METHODS：Curcumenol liposomes were prepared by mechanical dispersion method and ultrasonic method. MTT method was used to detect the inhibitory effects of Curcumenol and other drug on L1210 proliferation in mice with Lymphocytic leukemia .The apoptosis of L1210 cells induced by Curcumenol was observed under confocal microscope by trypan blue exclusion test and Hoechst 33258/PI double staining. RESULTS： Curcumenol significantly inhibited the proliferation of LI210 cells and induced apoptosis of L1210 cells. CONCLUSION： The antitumor effect of Curcumenol is possibly achieved by inducing the apoptosis of L1210 cells.