普通铁线蕨挥发油的HS-SPME-GC-MS分析

HS-SPME-GC-MS Analysis of the Essential Oil in Common Adiantum edgeworthii

目的:研究普通铁线蕨的挥发油化学成分。方法:采用顶空固相微萃取(HS-SPME)和气相色谱-质谱(GC-MS)联用技术对普通铁线蕨根和叶的挥发油成分进行分析和鉴定,用面积归一化法计算各成分的相对含量。结果:从根中鉴定出26个成分,从叶中鉴定出32个成分,两者中有20个成分是相同的。正壬醛可能是叶中主要的香气成分,2,6-二叔丁基对甲苯酚可能是根中主要的活性成分。结论:固相微萃取(SPME)技术操作简单、装置便于携带、操作成本低廉,适宜推广应用。

OBJECTIVE： To study the essential oil from Adiantum edgeworthii METHODS： The essential oil from Adiantum edgeworthii were analyzed and identified by head- space solid micro- extraction coupled with GC- MS （HS- SPME- GC- MS）, and the relative content of each constituent was derived by area normalization method. RESULTS： 26 constituents from the roots and 32 from the leaves of Adiantum edgeworthii were identified. 20 identical constituents were detected in both leaves and roots. N - nonanal might be the chief constituent in the leaves while 2,6- di - tert - butyl p - cresol the chief active constituent in the roots. CONCLUSION： The HS - SPME - GC - MS technology is characterized by simple operation, portable of the equipment involved, cheap in cost and its use deserves to be popularized.