HPLC法测定益心复脉颗粒中人参皂苷Rg1、Re、Rb1的含量

Determination of Ginsenoside Rb_1,Ginsenoside Re and Ginsenoside Rg_1 in Yixin Fumai Granula by HPLC

目的:建立以高效液相色谱法测定益心复脉颗粒中人参皂苷Rg1、Re、Rb1含量的方法。方法:色谱柱为Diamond C18(150mm×4.6mm,5μm),流动相为乙腈-水(梯度洗脱),流速为1mL·min-1,检测波长为203nm,柱温为25℃,进样量为10μL。结果:人参皂苷Rg1、Re和Rb1分别在0.645～6.450μg(r=0.999 8)、0.54～5.40μg(r=0.999 7)和0.605～6.050μg(r=0.999 9)范围内与各自峰面积积分值呈良好线性关系;三者平均加样回收率分别为100.59%(RSD=2.03%)、98.70%(RSD=1.46%)和98.99%(RSD=1.19%)。结论:本方法灵敏度高、简便、准确,可用于益心复脉颗粒的质量控制。

OBJECTIVE： To determine Ginsenoside Rg1, Ginsenoside Re and Ginsenoside Rb1 in Yixin fumai granula by HPLC. METHODS： Samples were determined on a Diamond Cls（150 mm × 4.6 mm, 5 μm）.The mobile phase consisted of accetonitrile- water by gradient elution with flow rate at 1 mL · min ^-1 UV detection wavelength at 203 nm, column temperature at 25 ℃ and sample size at 10 μL. RESULTS： The linear ranges of Ginsenoside Rgl,Ginsenoside Re and Ginsenoside Rbl were 0.645-6.450 μg（r = 0.999 8）, 0.54-5.40μg（r = 0.999 7） and 0.605-6.050 μg（r = 0.999 9）, respectively; the average recoveries were 100.59% （RSD = 2.03% ）, 98.70% （RSD = 1.46% ） and 98.99% （RSD = 1.19% ）, respectively. CONCLUSION： The method is sensitive, simple and accurate, and it can be used for the quality control of Yixin fumai granula.