HPLC法测定痰热清注射液中黄芩苷的含量

Determination of Baicalin in Tanreqing Injection by HPLC

目的:建立以高效液相色谱法测定痰热清注射液中黄芩苷含量的方法。方法:色谱柱为CLC-ODS C18(150mm×6.0mm,5μm),流动相为甲醇-水(47∶53),流速为0.5mL·min-1,检测波长为280nm。结果:黄芩苷检测浓度在2.53～12.65μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.999 8);平均回收率为99.61%,RSD=0.35%(n=9)。结论:本法简便、快速,可用于痰热清注射液的质量控制。

OBJECTIVE： To establish an HPLC method for the determination of Baicalin in Tanreqing injection. METHODS： The determination was performed by HPLC using an CLCODS C18 column（150 mm× 6.0 mm, 5 μm）, with mobile phase consisted of methanol- water（47 ： 53） at a flow rate of 0.5 mL · min^-1 and a detection wavelength of 280 nm. RESULTS： The calibration curve for Baicalin was linear in the concentration range of 2.53-12.65μg ·mL^-1（ r = 0.999 8）. The average recovery was 99.61% （RSD = 0.35%, n = 9） .CONCLUSION： The method is simple, rapid and applicable for the quality control of Tanreqing injection.