心身1号片对胃溃疡大鼠胃黏膜保护修复作用的探讨研究

Study on protectve and reparing effect of "Xinshenyihao" in mucosa of rats with gastric ulcer

[目的]研究心身1号片(XS-1)抗实验性大鼠胃溃疡的药理作用及机制。[方法]用浸水—束缚应激法和醋酸烧灼法分别制作胃溃疡模型,观察心身1号片对胃溃疡的保护修复作用和对胃黏膜凋亡相关基因B细胞淋巴瘤/白血病基因-2(BcL-2)和BcL-2相关蛋白(Bax)表达的影响。[结果]XS-1对大鼠水浸应激性胃溃疡的形成有显著预防的作用(P<0.01);对乙酸烧灼性溃疡有显著治疗作用(P<0.01)。两组模型中,XS-1组Bcl-2阳性细胞数目较造模组增多,两者具有明显差异(P<0.01);造模组Bax阳性细胞数目较心身1号片组增多,两者具有明显差异(P<0.01)。[结论]中药XS-1能够促进溃疡大鼠胃黏膜组织Bcl-2的表达,降低Bax的表达,这可能是XS-1抗溃疡的作用机制之一。

[Objective] To study the pharmacological action and mechanism of ＂Xinshenyihao＂（XS-1） in treating rats with experimental gastric ulcer. [Methods] Gastric ulcer was induced in rats by using soaking and restraining stress and acetic acid damage to study the function of XS-1 in protecting and repairing gastric ulcer, and the effect of XS-1 on the expression of Bcl-2 and Bax in rats with gastric ulcer was also determined. [Results] XS-1 played a significantly role in treating both acetic ulcer and in preventing stress ulcer. In both model groups, Bcl-2 positive cells in the XS-1 treated groups were significantly more than those in the model groups （P〈0.01）. In contrast,Bax positive cells in the model groups were significantly more than those in the XS-1-treated groups （P〈0.01）. [Conclusion] The traditional Chinese medicine Xinshenyihao can enhance the expresstion of Bel-2 and reduce the expresstion of Bax in mucosa of rats with gastric ulcer. This might be one of its mechanisms.