丙酮酸乙酯调节脓毒症小鼠肝细胞HSP70表达的研究

Modulation of hepatocyte heat shock protein 70 expression by ethyl pyruvate in septic mice

目的研究丙酮酸乙酯(EP)对脓毒症小鼠肝细胞热休克蛋白70(HSP70)的调节作用。方法制作小鼠盲肠结扎穿孔模型(CLP),应用丙酮酸乙酯林格氏液(REPS)与乳酸钠林格氏液(RLS)对小鼠进行液体复苏,60只小鼠分3组,各20只:假手术组、CLP模型+REPS复苏组、CLP模型+RLS复苏组,检测肝组织丙二醛(MDA)及肝细胞HSP70的表达。结果脓毒症小鼠较假手术组MDA浓度增高,P<0.01。EP显著提高脓毒症小鼠肝组织的抗氧化能力,REPS组肝组织MDA浓度低于RLS组([48.18±5.98)μmol/g.protvs(78.34±11.16)μmol/g.prot,P<0.01];REPS组小鼠肝细胞HSP70表达较RLS组增高[(28.76±5.69)vs(20.04±4.93),P<0.05]。HSP70表达与MDA值呈负相关(r=-0.733,P<0.01)。结论EP具有的抗氧化作用能提高脓毒症小鼠肝细胞的HSP70表达。

Objective To study the modulation of heat shock protein 70（HSP70） expression by ethyl pyruvate（EP） in the hepatocyte of septic mice. Methods Cecal ligation-perforation （CLP） models were made in mice as septic model. Total of 60 mice were divided into three groups： sham group, CLP＋REPS group and CLP＋RLS group. Ringer＇s ethyl pyruvate solution （REPS） and Ringer＇s lactic solution （RLS） were used in two groups to resuscitate the septic mice respectively. Malonaldehyde（MDA） in hepatic tissue and hepatocyte HSPTO expression was detected in different groups. Results Malondialdehyde level in septic groups was higher than in sham group（ P 〈 0.01）. EP promoted the anti-oxidative ability of hepatic tissue in septic mice. Malondialdehyde level was lower in REPS group than in RLS group[（48.18 ±5.98） μ mol ·g-lprotein vs （78.34 ± 1 1. 16） μ mol· g-lprotein, P〈0.01]. The expression of hepatocytic HSP70 in REPS group was higher than in RLS group [（28.76 ± 5.69） vs （20.04 ± 4.93）, P〈0.05]. The MDA level was negative correlated with the expression of HSP70 （r = -0.733, P〈0.01）. Conclusion Ethyl pyruvate, as its anti-oxidative ability, can increase the expression of HSP70 of hepatocyte in septic mice.