PCR法检测水中金黄色葡萄球菌肠毒素A基因

Study on the Detection of Staphylococcus aureus Enterotoxin A Gene in Water by PCR

[目的]建立一种快速准确检测水中金黄色葡萄球菌肠毒素A的方法。[方法]将金黄色葡萄球菌模拟污染的水增菌后提取菌体DNA,以沙门氏菌、大肠杆菌DNA和空白作对照,用PCR法扩增金黄色葡萄球菌肠毒素A基因。[结果]金黄色葡萄球菌DNA的检测结果呈阳性,检测底限达100 cfu/L,而大肠杆菌和沙门氏菌及空白对照均未出现特异性片段,整个检测过程只需要24 h。[结论]试验建立的PCR方法可检测水及类似食品中的金黄色葡萄球菌SEA基因,并具有特异性强、灵敏度高、速度快和易操作的特点。

[ Objective ] The aim was to establish a rapid specific assay for the detection of Staphylococcus aureu enterotoxin A in drinking water. [ Method] A polymerase chain reaction（PCR） assay targeting gene encoding Staphylococcus aureus enterotoxin A was developed for detecting Staphylococcus aureus in pure DNA and artificially contaminated water. Salmonella, E. coil DNA and blank were also amplified under the same condition. Staphylococcus aureus enterotoxin A was amplified by PCR. [ Result] The detection result of Staphylococcus aureus DNA was positive, with detection limit being 100 cfu/L; the while PCR products was not found in Salmonella, E, coli and blank. The total assay time including enrichment was approximately 24 h. [ Conclusion] Results suggested that the PCR could be used to rapidly detect Staphylococcus au- reus in water and possibly other types of food products, and the PCR assay also showed high specificity, sensitivity and easy operation.