椰子种质资源RAPD标记研究中的引物筛选

Primers Screening in the Study on Coconut Germplasm Resources with RAPD Markers

[目的]对椰子遗传多样性进行深入研究,为进一步开发利用椰子种质资源奠定基础。[方法]利用80个10碱基随机引物,对从中国海南、云南、广东、广西等椰子种植区所采集的67个椰子种质进行随机扩增,以筛选适合于对所有椰子品种进行RAPD分析的引物。[结果]共筛选出30条能扩增出条带清晰、明亮、具多态性且重复性好带型的有效引物。12个随机引物在所有样品中共扩增出340条带,即检测到340个椰子RAPD位点,其中307个位点为多态性位点,占总位点的90.3%。[结论]利用RAPD标记技术对椰子大量样品的系统分析,为椰子的品种鉴定、遗传育种及种质资源的保护与利用提供了依据和背景资料。

[ Objective ] The aim was to study the genetic diversity of coconut deeply in order to lay the foundation for the development and utilization of coconut germplasm resources. [ Method ] 67 coconut germplasm eoUeeted from coconut planting areas such as Hainan, Yunnan, Guangdong and Guangxi were used for random amplification by using 80 random 10-base primers to screen the suitable primers for RAPD analysis of all coconut varieties. [ Result] 30 effective primers amplifying clear and bright bands with polymorphism and good reproducibility were screened. 340 bands were amplified in all the samples with 12 random primers, which showed that there were 340 RAPD locidetected in the coconuts. Among these loci, there were 307 polymorphie loci, accounting for 90.3% of total. [ Conclusion ] The systems analysis on a great deal of coconut samples by using RAPD marking technology provided the basis and background information for variety identification, genetic breeding and the protection and utilization of germplasm resources.