一品红愈伤组织诱导·增殖及悬浮系建立的研究

Study on Poinsettia Callus Induction,Multification and Establishment of Suspension Cells

[目的]为一品红原生质体融合及遗传转化等相关研究奠定基础。[方法]以一品红品种“喜庆红”的顶芽和带芽茎段为外植体，进行愈伤组织诱导、增殖及悬浮系建立的研究。[结果]以浓度0．1％升汞处理顶芽和带芽茎段的时间分别以6min和10min为宜。在MS固体培养基里分别添加活性炭、PVP和Vc后，愈伤组织在培养过程中褐化程度有所减轻，但与对照相比差异并不显著。[结论]一品红愈伤组织悬浮系的适合继代周期为7～8d。

[ Objective ] The research aimed to provide foundation for the relative study of protoplast fusion and genetic transformation of poinsettia. [ Method ] Fresh stem segments with buds and shoot tipswith young leaves of poinsettia （ Eupgorbia pulcherrim） were used as explants in this study to explore callus induction, multiplication and establishment of the suspension cells. [ Result ] The results showed that the time of surface sterilization using HgC1 for poinsettia should be accepted according to the type of explants, and it was proven that shoot tips were treated by 0.1% HgC1 for 6 min, whereas stem segments with buds should be subjected to sterilization by 0.1% HgC1 for 10min. When active carbon, PVP or Vitamin C was added respectively to MS solid medium, the browning phenomenon began to decrease, but there was no significant difference comparing to that of the control. [ Conclusion ] 7 - 8 d was an appropriate culture cycle for establishment of poinsettia callus suspension cells.