病毒性脑炎患儿脑脊液NO及TNF-α的测定及其意义

The effect of detecting nitric oxide and tumor necrosis factor alpha in cerebrospinal fluid of viral encephalitis

目的探讨一氧化氮(NO)在病毒性脑炎发病中的作用及诊断价值。方法采用硝酸还原酶法及双抗体夹心ELISA法,分别测定20例病毒性脑炎(脑炎组)、15例高热惊厥(热惊组)及15例对照组患儿的脑脊液NO及肿瘤坏死因子α(TNF-α)水平。结果脑炎组NO及TNF-α浓度明显高于热惊组及对照组(P<0.01),热惊组与对照组比较无显著性差异(P>0.05)。结论NO确实参与了中枢神经系统的炎症过程;检测脑脊液中NO可以作为鉴别病毒性脑炎与高热惊厥的一项指标。

Objective To investigate the diagnosis value and effect of nitric oxide （NO） on pathogenesis in viral encephalitis of children. Methods 50 patients were involved ing the present study, including 20 cases with viral encephalitis children, 15 cases with febrile convulsion children （n= 15） and 15 normal children. NO in cerebrospinal fluid （CSF） was measured with nitric-acid reductase. Tumor necrosis factor alpha （TNF-α） in CSF was detected with enzyme-linked immunosorbent assays （ELISA）. Results The level of No and TNF-α in CSF in viral encephalitis group were higher than that in febrile convulsion group and control group （P〈0. 01）. However, there was no significant difference between febrile convulsion group and control group. Conclusion It is certain that NO plays a role in pathogenesis of viral encephalitis. NO level in CSF may be a sign to distinguish between viral encephalitis and febrile convulsion.