几种去除病理教学标本肺内组织液染色前方法的比较

Several methods comparisons of processing the pathological teaching specimen in eliminating intra pulmonary tissue fluid before dyeing

目的:病理教学切片的制作与常规切片制作有所不同,病理实验是加深和巩固提高病理理论知识的基础。为达到充分展示实验教学切片标本典型病变特征,染色前用几种方法处理标本,去除肺泡内组织液,获得满意的结果。方法:采用胰蛋白酶法、4%NaOH、3%NaOH、2%NaOH、胰蛋白酶pH法等方法。结果:胰蛋白酶pH法对肺泡内的组织液前期消化处理效果最好。结论:胰蛋白酶pH法处理的标本,切片背景干净,病变处组织细胞完整不变形,组织结构清晰;无水肿液覆盖肺泡表面及支气管腔面,小叶性肺炎病变特征非常明显。

Objective Sincee manufacture of pathological teaching specimen is different from the Convention. Pathology experiment is the foundation of consolidating and enhancing theoretical knowledge. Several special processing methods were used to display the typical characteristic of experimental slide. Methods Parenzyme; 4% NaOH; 3% NaOH; 2% NaOH and Parenzyme pH methods were used. Results Parenzyme pH shift is the best method to digest intra pulmonary tissue fluid. Conclusion The background of the slide is clear,with tissues and cells complete and in original shape. No tissue fluid overlay the surface of pulmonary, alveoli and the lumen of bronchi,the pathological characteristic of lobular pneumonia is apparente.