深圳地区婴幼儿杯状病毒肠炎流行病学调查

Molecular Epidemiology of Human Caliciviruses Diarrhea among Infants and Young Children in Shenzhen Area

目的:研究深圳地区婴幼儿感染杯状病毒所致肠炎的流行病学特点。方法:在我院采集的腹泻患儿的粪便标本2354份,进行轮状病毒(RV)、杯状病毒(HuCV)、星状病毒(AstV)检测分析。结果:2354份粪便标本中,RV阳性910份,阳性率为38.7%。G血清型检测355份标本,其中以G3型为主,占57.5%(204/355);对1444份RV阴性标本进行HuCV、AstV和AdeV检测,检出HuCV阳性511份,阳性率为35.4%;AstV阳性342份,阳性率为23.7%;检出AdeV阳性49份,阳性率为3.4%。结论:轮状病毒、杯状病毒是深圳地区婴幼儿感染杯状病毒所致肠炎的主要病原体。

Objective To investigate the characteristics of human caliciviruses （HuCV） diarrhea among infants and young children with acute diarrhea in Shenzhen area. Methods Stool specimens were collected from outpatients and inpatients with acute diarrhea. ELISA and PCR were used to detect rotavirus （RV）, human caliciviruses （HuCV）, astrovirus （AstV） and Adenovirus （AdeV）. RT-PCR was used to detect HuCV in stool specimens of RV ELISA negative. Results Among 2354 speimens, 910 was positive with RV（ 38.7% ）. Among 355 speimens of rotavirus G tested by serotyping, G3 was the predominated type （57.5%） ;511 of the 1444 negative specimens of RV were HuCV positive results （35.4%）, 342 with AstV posi- tive results （23.7%）, and 49 with AdeV positive results （3.4%）. Conclusion HuCV is one of the major etiological agents of viral diarrhea among infants and young children in Shenzhen area. HuCV, AstV and AdeV are the important etiological agents.