人热休克蛋白60及其自身抗体与冠心病的相关性

Correlation and clinical significance among coronary heart disease and human heat shock protein 60 and its autoantibody

目的:探讨人热休克蛋白60(HSP60)及其自身抗体(HSP60 Ab)与冠心病的关系。方法:从我院2007年1月到2007年8月心内科住院患者中入选96例研究对象,分为冠心病(CHD)组[包括急性冠脉综合征(ACS)组、稳定性心绞痛(SAP)组]、胸痛综合征(CPS)组、正常对照组(各组性别、年龄,高危因素等匹配)。用ELISA方法分别测定血清HSP60和HSP60 Ab浓度。结果:HSP60水平:CHD组的明显高于CPS组和对照组(P<0.01);ACS组的明显高于SAP组(P<0.01);SAP组,CPS组和对照组之间差别无显著性(P>0.05);血清HSP60 Ab水平:CHD组的明显高于CPS组和对照组(P<0.01),ACS组明显高于SAP组(P<0.01),SAP组的明显高于对照组和CPS组(P<0.01),对照组和CPS组的差别无显著性(P>0.05);血清HSP60与HSP60 Ab水平呈正相关(r=0.805,P<0.01),均为预测ACS的独立危险因素(β=0.785,P<0.01)。结论:血清HSP60、HSP60 Ab水平与冠心病有明显相关性,可能在冠心病及急性冠脉综合征的发病过程中起着相互协同作用,是预测急性冠脉综合征的独立危险因素。

Objective： To investigate the correlation among coronary heart disease （CHD） and serum heat shock protein 60 （HSP60） and its autoantibody （HSP60 antibody）. Methods： A total of 96 subjects including 40 patients with acute coronary syndrome （ACS）, 24 patients with stable angina pectoris （SAP）, 16 patients with chest pain syndrome （CPS） and 16 healthy adult as controls were selected from our hospital （gender, age and other risk factors were paral- lel in every group）. The levels of Hu-- HSP60 and HSP60 Ab were determined by enzyme linked immunosorbent assay （ELISA）. Results.. The serum Hu--HSP60 levels in patients with CHD （including ACS and SAP） were significantly higher than those in CPS group and controls （P〈0. 01）; serum HSP60 level in ACS group was significantly higher than that in SAP group （P〈0.01）, whereas there was no significant differences among SAP, CPS and control group （P〉0. 05）. The serum levels of Hu-- HSP60 Ab in CHD patients was significantly higher than that in CPS group and control group （P〈0.01）; The serum HSP60 Ab level in ACS group was significantly higher than that in SAP group （P〈0.01） and there was no significant differences between CPS group and control group （P〉0.05）. There was a positive correlation between the serum HSP60 level and the serum HSP60 Ab level （r=0. 805, P〈0.01）, and they were independent risk factors of ACS respectively （β=0. 785, P%0.01）. Conclusion： The serum Hu-- HSP60 and HSP60 Ab levels are significantly correlated to the severity of CHD, probably participated in the autoimmune response promoting the formation of atherosclerosis and destabilization of plaque. They may be independent risk factors for predicating ACS.