拜阿司匹林对小鼠主动脉核转录因子-κB与基质金属蛋白酶-9表达的调控

Effect of β-Aspirin on matrix metalloproteinase-9 and nuclear factor kBp65 expression in aortic artery of atherosclerostic mouse

目的:观察拜阿司匹林对小鼠主动脉核转录因子-κB(NF-κB)与基质金属蛋白酶-9(MMP-9)表达的调控作用。方法:40只13周龄小鼠分为拜阿司匹林组(给高脂饲料,拜阿司匹林6mg/kg灌胃),氟伐他汀组(给高脂饲料,氟伐他汀3mg/kg灌胃),模型组(给高脂饲料,生理盐水1ml灌胃),正常对照组(给予普通饲料,生理盐水1ml灌胃),每组10只,17周后,取主动脉,以免疫组化法观察其NF-κB和MMP-9表达的程度。结果:较之对照组模型组,小鼠主动脉粥样斑块的NF-κB与MMP-9表达明显增加,拜阿司匹林组和氟伐他汀组均显著降低其表达(P均<0.01),拜阿司匹林组和氟伐他汀组间其表达水平无显著差异(P>0.05)。结论:拜阿司匹林可以降低NF-κB与MMP-9的表达,从而抑制血管内炎症反应和基质纤维蛋白的降解,起到治疗动脉粥样硬化的作用。

ObjectiveS. To study the effects ofβ--aspirin on expression of matrix metalloproteinase--9 （MMP--9） and nuclear factor κB （NF--κB）. Methods.. Forty mouse with the age of thirteen weeks were averagely divided into normal control group, model group, β--aspirin group, fluvastatin group. The mouse of model group, β--aspirin group, fluvastatin group were received the high lipid diet, and the normal control group received the normal diet. In the same time, the β--aspirin group was treated with aspirin intragastric administration 6 mg/kg; the fluvastatin group with fluvastatin intragastric administration 3 mg/kg. Seventeen-week after treatment, expression of MMP--9 and NF--κB in aorta was detected by immunohistochemistry. Results： Compared with normal control group, the expression of MMP --9 and NF--κB significantly increased （P〈0.01） in model group. In β--aspirin group and fluvastatin group the expression of MMP--9 and NF--κB significantly decreased compared with those of model group （P〈0.01）. The expression of MMP--9 and NF-κB was no difference between β--aspirin group and the fluvastatin group （P〉0.05） Conclusion. β-- aspirin can inhibits the expression of MMP-- 9 and NF-- κB, so may increases the stability of plaques;