他克林对急性胰腺炎大鼠血清细胞因子的影响研究

The research on how tacrine affect the cytokine level in laboratory rats with acute pancreatitis

目的探讨静脉注射他克林对急性胰腺炎大鼠细胞因子的影响。方法健康Wistar大鼠108只，随机分为假手术组、对照组、他克林组各36只，分别测定三组动物模型建立成功后1h、12h、24h、36h、48h、72h血清中TNF-α、IL-6、IL-10的含量变化。结果假手术组各细胞因子均处于较低水平，对照组较假手术组各个时段细胞因子水平都明显升高（P〈0．05），而他克林组大鼠细胞因子TNF—α、IL-6在1h、12h、24h、36h、48h、72h时也明显较假手术组升高（P〈0．05），但是其升高幅度与对照组相比明显降低（P〈0．05）。他克林组保护性细胞因子IL-10在发病初1小时有升高，与对照组相比P：0．933；而在12～72h，IL-10升高明显，并逐步达到一定高度，与对照组相比有显著性差异（P〈0．05）。结论静脉注射他克林能够降低胰腺炎大鼠血清损伤性细胞因子TNF—α、IL-6的释放，升高保护性细胞因子IL-10水平。

Objective To investigate how the cholinergic anti-inflammatory pathway influncing the cytokine level in laboratory rats with pancreatitis. Methods 108 Wistar rats were randomly divided into sham group （SG）, control group （CG） and taerine injection group（TG）, serum cytokines content variation of TNF-α, IL-6, IL-10 in different period were determined. Results Serum concentrate of cytokines in SG keep a low level, but what in CG were higher than that in SG, which is signifficant statistically（ P 〈0. 05）. The concentrate of TNF-α, IL-6 in TG were higher than that in SG,in each period（ P d0.05）. But much lower than that in CG （ P〈0.05）. Serum level of IL-10 in TG start rising in 1 hour, but it has no signifficance comparing to control group（ P = 0. 933）. At 12-72h, the IL-10 level rised to a high level in TG,which was higher than in CG （ P d0.05）. Conclusion Tacrine intravenous injection could increased serum protective eytokine IL-10 level, but decreased injury cytokines TNF-α,IL-6 content in laboratory rats with pancreatitis.