信号转导和转录激活因子4及γ-干扰素在银屑病患者皮损中的表达及其意义

Expression and significance of STAT4 and interferon gamma in patient with psoriasis

目的研究银屑病患者皮损内信号转导和转录激活因子4(STAT4)及γ-干扰素(IFN-γ)的表达及其临床意义。方法利用荧光定量聚合酶链反应法测定20例寻常型银屑病患者皮损内及17例正常对照者皮肤中STAT4 mRNA及IFN-γmRNA的定量表达,采用直线相关回归分析方法分析STAT4mRNA及IFN-γmRNA的表达与银屑病病情严重指数(PASI)的相关性,STAT4mRNA与IFN-γmRNA表达的相关性。结果银屑病皮损内STAT4mRNA及IFN-γmRNA的表达均高于正常对照者皮肤内的表达水平(P<0.001)。银屑病皮损内STAT4mRNA的表达与患者PASI呈显著的正相关(r=0.78465,P<0.0001),IFN-γmRNA的表达与患者PASI无显著相关性(r=-0.16125,P=0.4970)。银屑病皮损内STAT4mRNA的表达与IFN-γmRNA的表达呈显著的正相关(r=0.62149,P=0.0034)。结论STAT4可能通过上调IFN-γ的表达,促进银屑病的炎症进程。

Objective To study the expression and significance of signal transducers and activators of transcription 4 （STAT4） and interferon gamma （IFN-γ）in psoriatic skin lesion. Methods Twenty adult patients with vulgaris psoriasis and 17 health control were sequentially enrolled in this study. The production STAT4 mRNA and IFN-γmRNA was detected by real-time polymerase chain reaction. The relationship between STAT4 and disease severity （PASI） was assessed by correlation analysis. The relationship between IFN-γ and PASI was assessed by correlation analysis. The relationship between STAT4 rnRNA and IFN-γmRNA was assessed by correlation analysis. Results Expression of STAT4 mRNA and IFN-γmRNA in psoriasis lesional skin was higher than that in control subject （ P〈0. 001）. Expression of STAT4 mRNA was markedly positive correlation with PASI（ r = 0. 78465, P d0. 0001）. Expression of IFN-γ mRNA was negative correlation with PASI（ r =0. 16125, P =0. 4970）. Expression of STAT4 mRNA was markedly positive correlation with IFN-γmRNA （ r =0. 62149, P = 0. 0034）. Conclusion Over expression of IFN-γ might be regulated by STAT4. STAT4 might play an important role of in the development and advance of psoriasis.