高效液相色谱法测定猪肌肉中乙酰甲喹的残留量

Determination of Mequindox Residues in Pork by High Performance Liquid Chromatography

建立了猪肌肉中乙酰甲喹残留量的高效液相色谱检测方法．试样用双蒸水提取，三氯乙酸沉淀蛋白质，经C18固相萃取小柱净化，用高效液相色谱检测．选择甲醇-水（40：60，V：V）为流动相，流速为0．7mL／min，在373nm波长处检测．该方法检测的乙酰甲喹在0．040～2．000mg／L范围内线性良好，相关系数大于0．999；在50、100、400μg/kg3水平加标平均回收率在71．1％～77．4％之间，批内变异系数为3．8％～5．4％，批间变异系数为2．8％～7．4％；乙酰甲喹在猪肌肉中的检出限达16μg/kg，定量限为50μg/kg．

A method for the determination of mequindox residues in pork by high performance liquid chromatography（HPLC） is reported. The composite sample was extracted with warm water and trichloro acetic acid, centrifuged, and the supernatant was applied to LC-18 SPE cartridge successively. The drug was eluted from the cartridge with 80% methanol, and the eluate was detected by HPLC with the following conditions： the mobile phase consisted of methanol-deionized water（40：60, V： V）, and flowed at a rate of 0. 7 mL/min, wavelength of UV detector at 373 nm. The range of linearity was 0. 040 - 2. 000 mg/L, and relevant coefficient was more than 0. 999. Recoveries from pork fortified at 50, 100 and 400 μg/kg were between 71.1% and 77.4%, with all the relative standard deviations of less than 8%, and LOD of the method was 16 μg/kg, LOQ of pork was 50 μg/kg.