酵母扩培的实验室阶段

Lab Stage of Expanding Culture of Beer Yeast

啤酒酵母的纯种培养在啤酒生产中非常重要,酵母扩培实验室阶段的技术要点:①扩培人员要有强烈的责任感和扎实的工作经验,严格保证扩培过程无菌;②采用普通斜面、4℃冰封保藏菌种;③选择优良的培养基;④保证扩培过程中的充氧量;⑤转接过程中选择适当的温度及合理的扩大比例。

The pure species culture of beer yeast is of vital importance in beer production. The technical points of expanding culture of beer yeast in lab stage were as follows： 1. technicians must have strong sense of responsibility and solid foundation of working experience, and aseptic culture is required in expanding culture; 2. common inclined plane is adopted, and ice-sealed storage of microbial species at 4 ℃ required; 3. selection of quality culture medium; 4. oxygenation capacity should be guaranteed in expanding culture process; 5. appropriate temperature and rational expanding culture proportion selected during transferring process. （Tran. by YUE Yang）