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PCR-DGGE技术及其在食品行业中的应用 被引量：7

PCR-DGGE & Its Application in Food Industry
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摘要 现代分子生物学技术PCR-DGGE是一种分析微生物群落的有效工具,可以用于研究生态系统中微生物多样性和群落动态性。简要介绍了PCR-DGGE技术原理及其在食品行业中微生物生态学领域的应用。 As a modern molecular biology technique, PCR-DGGE is a powerful tool for the analysis of microbial populations,which could be used to study the diversity and dynamics of microbial populations. The technical principles of PCR-DCGE and its application in food industry （microbial ecology） were introduced briefly in this paper.
作者 梁慧珍 崔丽华 张春辉 赵树欣
机构地区 天津天士力集团有限公司食品研究所 北京师范大学图书馆 天津科技大学生物工程学院
出处 《酿酒科技》 北大核心 2008年第10期89-91,共3页 Liquor-Making Science & Technology
关键词 PCR—DGGE 微生物群落动态性 食品行业 应用 PCR-DGGE microbial population dynamics food industry application
分类号 TS207 [轻工技术与工程—食品科学]
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参考文献20

  • 1Fischer S G, Lerman L S. DNA fragments differing by single basepair substitutions are separated in denaturing gradient gels correspondence with melting theory[J]. Proceedings of the National Academy of Science of USA. 1983,80: 1579-1583.
  • 2Muyzer, G., Ellen, C.D. W, Andre, G.U. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction genes coding for 16S rRNA[J]. Applied and Environmental Microbiology. 1993,59 : 695-700.
  • 3Ferris M J, Muyaer G, Ward D M. Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined population inhabiting a hot spring microbial mat community[J]. Applied and Environmental Microbiology. 1996,62:340-346.
  • 4Casamayor E O, Achafer H, Baneras L, et al. Identification of and spatiotemporal differences between microbial assemblages from two neighboring sulfurous lakes: comparison by microscopy and denaturing gradient gel electrophoresis[J].Applied and Environmental Microbiology.2000,66:499-508.
  • 5Sekiguchi H, Watanabe M, Nakahara T, et al. Succession of bacterial community structure along the ChangJiang River determined by denaturing gradient gel electrophoresis and clone library analysis[J]. Applied and Environmental Microbiology. 2002,68 : 5142-5150.
  • 6Bano N, Hollibaugh J T. Phylogenetic compostion of bacterioplankton assemblage from the Arctic ocean[J]. Applied and Environmental Microbiology. 2002,68 : 505-518.
  • 7Duineveld B M, Rosado A, Elsas J D, et al. Analysis of the dynamics of bacterial communities in the rhizophere of the chrysanthemum via denaturing gradient gel electrophoresis and substrate utlization patterns[J]. Applied and Environmental Microbiology. 1998,64:4950-4957.
  • 8Smit E, Leeflang P, Gommans S, et al. Diversity and seasonal fluctuations of the dominant members of the bacterial soil community in a wheat field as determined by cultivation and molecular methods[J]. Applied and Environmental Microbiology. 2001,67 : 2284-2291.
  • 9Kowalchuk G A, Stephen J R, de Boer W, et al. Analysis of ammonia-oxidizing bacteria of the B-subdivision of the class proteobacteria in coastal sand dunes by denaturing gradient gel electrophoresis and sequencing of PCR-amplified 16S rDNA fragments[J]. Applied and Environmental Microbiology. 1997,63 : 1489-1497.
  • 10Ampe F, Ben Omar, N Moizan, C Wacher, C Guyot J-P. Polyphasic study of the spatial distribution of microorganisms in Mexican pozol, a fermented maize dough, demonstrates the need for cultivation-independent methods loinvestigate traditional fermentations[J].Appl Environ Microbiol 1999,65 : 5464-5473.

二级参考文献13

  • 1陈建平,胡孝素,杨文天.杜氏利什曼原虫SSU　rRNA逆转录多聚酶链反应的建立[J].实用寄生虫病杂志,1996,4(1):1-3. 被引量：3
  • 2Muyzer G,De Waal E C,Uitterlinden A G.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA[J].Appl Environ Microbiol,1993,59:695～700.
  • 3William G,et al.J Bacteriol,1991,173:697～703.
  • 4Ampe F,Ben Omar,N Moizan,C Wacher,C Guyot JP.Polyphasic study of the spatial distribution of microorganisms in Mexican pozol,a fermented maize dough,demonstrates the need for cultivation-independent methods to investigate traditional fermentations[J].Appl Environ Microbiol,1999,65:5464～5473.
  • 5Meroth C B,Walter J,Hertel C,Brandt M J,Hammes W P.Monitoring the bacterial population dynamics in sourdough fermentation processes by using PCR-denaturing gradient gel electrophoresis[J].Appl Environ Microbiol,2003,69:475～482.
  • 6Ercolini D,Hill P J,Dodd C E R.Development of a fluorescence in situ hybridisation method for cheese using a 16S rRNA probe[J].J Microbiol Methods,2003,52:267～271.
  • 7Randazzo C L,Torriani S,Akkermans A D L,de Vos W M,Vaughan E E.Diversity,dynamics and activity of bacterialcommunities during production of an artisanal Sicilian cheese as evaluated by 16S rRNA analysis[J].Appl Environ Microbiol,2002,68:1882～1892.
  • 8Cocolin L,Bisson L F,Mills D A.Direct profiling of the yeast dynamics in wine fermentations[J].FEMS Microbiol Lett,2000,189:81 ～87.
  • 9Mills D A,Johannsen E A,Cocolin L.Yeast diversity and persistence in Botrytis-affected wine fermentations[J].Appl Environ Microbiol,2002,68:4884～4893.
  • 10Van Beek S,Priest F G.Evolution of the lactic acid bacterial community during malt whisky fermentation:a polyphasic study.Appl[J].Environ.Microbiol,2002,68:297～305.

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二级引证文献38

酿酒科技
2008年 第10期

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