啤酒中嘌呤类物质的测定研究

Determination of PurineCompounds in Beer

建立了高效液相色谱法测定啤酒中嘌呤类物质含量的分析方法。啤酒中的嘌呤类物质经高氯酸水解成嘌呤碱,其优化水解条件为:啤酒∶高氯酸为1∶1,100℃水解30min。优化色谱条件为:采用Eclipse XDB-C18反相色谱柱,以0.02mol/L的KH2PO4-H3PO4的缓冲溶液(pH4.00)为流动相,流速1.0mL/min,检测紫外波长为210.4nm,柱温为25℃。结果表明,在优化的色谱条件下,4种嘌呤碱的平均加标回收率为92.91%～102.48%,方法精密度为4.51%～5.47%。该方法的灵敏度高、操作简便、快速,能够满足啤酒中嘌呤类物质的高灵敏分析。

The analytic method （HPLC） of purine compounds content in beer was developed. The purine compounds in beer were prehydrolyzed to purines by perchlorate acid and the optimized hydrolysis conditions were as follows： the ratio of beer to perchlorate acid was 1：1, and 30 min hydrolysis at 100 ℃. The optimized chromatography conditions were the following： Eclipse XDB-C18 RP-column, 210.4 nm by UV detector with 0.02 mol/L of KH2PO4-H3PO4 buffer solution （pH = 4.00） as mobile phase, and flow rate at 1.0 mL/rain, and the column temperature was at 25 ℃. The results showed that under the optimized chromatography conditions, the average recoveries of four purine bases were 92.91% 102.48 %, and the accuracy of the method as RSD of all four purine bases were 4.51%--5.47 %. This method had the advantages including high sensitivity and simple and fast operation and it could be used for high sensitive analysis ofpurine compounds in beer.