海带蛋白的提取和活性研究

Isolation and Determination of Biological Activities of Proteins in Kelp Laminaria japonica

采用5％NaOH、0．15mol／L NaCl、0．1mol／L磷酸盐缓冲液、0．15mol／L NaCl及0．06％纤维素酶和1％SDS溶液提取新鲜海带中的蛋白质，并采用Brrddford的方法测定提取的蛋白质含量。结果表明，采用5％NaOH＋0．06％纤维素酶浸泡，然后经超声波破碎，离心取上清液进行100％饱和度的（NH4）2SPO4沉淀，对所得沉淀透析后冻干所得海带蛋白质的含量较多。时间飞行质谱表明蛋白质分子量为872～7705m／z。抑菌试验表明海带蛋白对大肠杆菌、产气杆菌、金色葡萄球菌具有抑制作用。同时，以海带蛋白（5mg／kg）对高血压模型大鼠灌胃具有良好的降压作用。

The proteins were isolated from kelp Laminaria japonica by 5% NaOH, 0.15 mol/L NaCl, 0.1 mol/ L PB, cellulase（0.06% ）and 1% SDS. The protein content of the kelp samples was determined by Brrddford＇s method. More proteins was isolated from the kelp by the process of immersion in 5% sodium hydroxide and cellulase （0.06%）, followed by ultrasonic fracture and addition of pulverized ammonium sulphate to the supernatant up to 100% saturation. MALDI-TOF showed that the proteins from the kelp had molecular weight of 872 - 7705 m/z. The antibiotic test showed that the proteins had antibacterial activity of Escherichia coli, Enterobacter aerogenes and Staphylococcus aureus, and better antihypertensive effects on renovascular hypertensive rats.