酒泉地区婴幼儿腹泻的A组轮状病毒分子基因特征研究

Genetic Profile of Rotavirus A Among the Acute Diarrhea Infants in Jiuquan Region

目的采用基因分型方法调查研究酒泉地区婴幼儿A组轮状病毒(rotavirus,RV)腹泻的基因分型特点。方法对2001年11月至2002年12月酒泉地区收集的腹泻婴幼儿324份粪便标本,电脑随机抽样选取204份采用酶联免疫吸附试验(enzyme-linked immunosorbant assay,ELISA)和逆转录-聚合酶链反应(RT-PCR),进行轮状病毒病原检测,扩增A组轮状病毒VP4和VP7基因,并对阳性标本进行基因分型。结果酶联免疫吸附试验法测得204份粪便标本的A组轮状病毒阳性率为54.9%(112/204)。对酶联免疫吸附试验呈阳性的标本进行逆转录-聚合酶链反应法检测A组轮状病毒G,P基因型,结果表明,酒泉地区流行的A组轮状病毒主要基因型为G3P[8]型(50.0%),其余依次为G1P[4](15.8%)、G3P[4] (15.8%)、G1P[8](10.6%)、G2P[4](2.6%)和G1G3P[4](2.6%)、G1G3P[8](2.6%)。结论A组轮状病毒是酒泉地区婴幼儿非细菌性腹泻的主要病原,其中G3P[8]型是主要基因型。

Objective To investigate genetic subtypes of rotavirus A prevailed among acute diarrhea infants in Jiuquan, China. Methods From November 2001 to December 2002, 204 stool specimens collected from both outpatients and inpatients diagnosed as acute infant diarrhea, were tested by enzyme-linked immunosorbant assay （ELISA） and reverse transcription-polymerase chain reaction （RT- PCR） to identify the rotavirus infection and subtypes respectively, at the Department of Pediatrics, the People＇s Hospital of Jiuquan. There were 14 gene loci in total of the two rotavirus genes, rotavirus A VP4 and VPT, tested in this research to uncover the major genotypes on the G and P subtypes. Results Enzyme-linked immunosorbant assay results showed that rotavirus A was present in 112 cases out of 204 specimens （54. 9%） without gender priority. G type accounted for 82 cases from the ELISA positive samples, which had G1,G2, G9, G1G2 and G1G3 subgroups. Among randomly selected 53 G-positive genotype specimens, 38 cases were subtyped as P strain, including P[4] and P[8], and the remaining 15 cases lacked the marker for the P strain. The overlap analysis of G and P subtype for rotavirus A showed that G3P[8] was the dominant （50.0%） type, followed by G1P[4] （15.8%）, G3P[4] （15.8%）, G1P[8] （10. 6%）, G2P[4] （2. 6%）, G1G3P[4] （2. 6%）, G1G3P[8] （2. 6%） sequentially. Conclusion Rotavirus A was the major pathological agent of the infant and young children diarrhea in Jiuquan,a region where the predominant rotavirus stain was found out to be G3P[8].