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兔卵母细胞体外成熟过程中生长分化因子9基因的表达 被引量：1

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摘要 本研究参考人、小鼠、羊等物种的GDF9基因序列设计引物,利用RT-PCR法扩增兔GDF9部分cDNA序列,实时荧光定量PCR法检测该基因在兔卵细胞体外成熟过程中的表达变化。结果获得兔GDF9基因289bp的部分cDNA序列,与人、猪、山羊相对应序列的同源性分别为81%、79%、76%。在兔卵母细胞体外成熟过程中,GDF9的表达是呈动态变化的,以0h为对照,8h表达量急剧下降,为0h的0.0124倍,16h和24h表达量分别为0h的1.4559倍和1.9096倍。
作者 季金强 尹萍 李霖 丁家桐
机构地区 扬州大学动物科学与技术学院
出处 《上海畜牧兽医通讯》 2008年第5期25-27,共3页 Shanghai Journal of Animal Husbandry and Veterinary Medicine
关键词 卵母细胞 生长分化因子9 实时荧光定量PCR
分类号 S829.1 [农业科学—畜牧学]
  • 相关文献

参考文献15

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二级参考文献16

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共引文献4

同被引文献14

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  • 6ECKERY D C ,WHALE L J,LAWRENCE S B ,et al. Expression of mRNA encoding growth differentiation factor 9 and bone morphogenetic protein 15 during follicular forma-tion and growth in a marsupial,the brushtail possum (Trichosurus vulpecula) [J]. Mol Cell Endocrinol ,2002,192:115 -126.
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上海畜牧兽医通讯
2008年 第5期

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