血小板衍生生长因子及其受体在人及小鼠神经肌肉接头的表达研究

Expression of platelet-derived growth factor and its receptor at human and mouse neuromuscular junctions

目的探讨血小板衍生生长因子及其受体在正常人和小鼠肌肉组织神经肌肉接头的表达及其对神经肌肉接头功能的调节作用。方法应用特异性血小板衍生生长因子-A及其α受体多克隆抗体，检测正常人和小鼠神经肌肉接头血小板衍生生长因子及其α受体的表达；对正常人肌肉标本连续切片进行血小板衍生生长因子和乙酰胆碱受体免疫染色；用双重荧光免疫方法进行血小板衍生生长因子及其α受体和乙酰胆碱受体的双重定位。结果血小板衍生生长因子及其α受体分别定位于人和小鼠的神经肌肉接头，与乙酰胆碱受体紧密合一。结论血小板衍生生长因子及其α受体积聚于人和小鼠的神经肌肉接头，血小板衍生生长因子可能参与突触前后组分之间的相互作用。对正常及疾病肌肉的神经肌肉接头处信号传导发挥作用。

Objective To investigate the immunoreactivity of platelet-derived growth factor-A （PDGF-A） and its receptor at human and mouse neuromuscular junctions and to explore their regulatory effects on neuromuscular junctions. Methods Specific polyclonal antibodies were used to detect PDGF^2-A and PDGF a-receptor expression. Double immunohistochemistry for platelet-derived growth factor and the acetylcholine receptor was performed on normal human muscle biopsy specimens. Double fluorescence labeling was applied to detect immunoreactivity for PDGF-A, its receptor and acetylcholine receptors. Results PDGF-A and its receptor was closely co-localized with acetylcholine receptors at human and mouse neuromuscular junetions. Conclusion PDGF-A and its receptor concentrated at human and mouse neuromuscular junctions. PDGF might be involved in the interaction between the presynaptic and postsynaptic components. PDGF-A and its receptor might play regulatory role in signaling at neuromuscular junctions in normal muscle.