肺鳞癌抗癌基因P53突变与人孔头状瘤病毒感染的分析

ASSOCIATION BETWEEN MUTATION OF P53 GENE AND HUMAN PAPILLOMAVIRUS INFECTION IN SQUAMOUS CELL CARCINOMA OF THE LUNG

本实验收集经病理确诊为肺鳞癌的标本67例，应用免疫组织化学技术检测到P53蛋白的阳性率为61．2％。同时结合聚合酶链反应检测人乳头状瘤病毒（HPV）感染，检出感染标本10例（14．9％），其中4例伴有P53蛋白阳性。表明肺鳞癌中存在着HPV感染。本文结果也提示，P53基因突变与肺鳞癌的分化程度无联系。

Sixty seven specimens of bronchial squamous cell carcinoma were obtained from histopatho logically identified cases. P53 protein was detected in forty-one of 67 specimens(61. 2%) by im munohistochemistry; human papillomaviruses(HPVs) infection was detected in ten of 67 speci mens(14. 9%) by polymerase chain reaction (PCR ) ; and in these 10 cases of HPV infection, 4 cases had P53 protein positive expression. Results showed that a high proportion of bronchial squamous cell carcinomas had P53 gene mutation and some had HPV infection,and that P53 gene mutation was not associated with the differentiated degree of bronchial squamous cell carcinoma