摘要
目的建立HPLC法测定维吾尔药醒脑库克亚片中芦荟苷含量的方法。方法色谱柱为Shimadzu ODS VP(4.6mm×250mm,5μm);流动相为乙腈-水(25:75);流速为1.0ml/min;检测波长为360nm;柱温:30℃。结果芦荟苷进样量在0.238-2.38℃g范围内呈良好的线性关系(r=0.9999),平均加样回收率为98.80%,RSD为2.0%(n=5)。结论本方法操作简单、结果准确、重复性好,可用于该制剂的质量控制。
Aim To develop a method for determination of barbalion in Xingnaokukeya tablets of Uygur medicine. Methods HPLC with Shimadzu ODS VP (4.6mm×250mm,5μm)column was used. The mobile phase consisted of aceto-nitrile-water (25: 75) ,with a flow rate of 1.0ml/min and detection wavelength at 360nm and under 30℃. Results The linear range was 0.238-2.38μg( r =0.9999). The average recovery ( n = 9)was 98.80 % and RSD was 2.03%. Conclusion The method is simple ,accurate,sensitive and could be used for quality control of the preparation.
出处
《解放军药学学报》
CAS
2008年第5期444-445,共2页
Pharmaceutical Journal of Chinese People's Liberation Army